Generate Size-Controlled Spheroids and Embryoid Bodies Using AggreWell™ Microwell Plates
AggreWell™ plates provide an easy and standardized approach to control size of embryoid bodies (EBs) for differentiation by adjusting initial cell input
AggreWell™ microwell plates provide an easy and standardized approach to generate 3D cell aggregates of a defined size. Cell aggregates can be generated from many cell types, including cancer cell lines for tumor spheroid formation, and human pluripotent stem cells (PSCs) for embryoid body (EB)-based differentiation protocols. Different AggreWell™ plate formats are available to suit your particular requirements.
|
|
AggreWell™400 |
AggreWell™800 |
AggreWell™HT |
|||
|
Microwell size (diameter) |
400 µm |
800 µm |
900 µm |
|||
|
Aggregate size range (# of cells per microwell) |
50 - 3,000 |
3,000 - 20,000 |
50 - 20,000 |
|||
|
Plate format |
24-well |
6-well |
24-well |
6-well |
96-well |
|
|
Number of aggregates generated per well |
~ 1,200 |
~ 5,900 |
~ 300 |
~ 1,500 |
~ 32 |
|
|
Total number of aggregates per plate |
~ 28,000 |
~ 35,400 |
~ 7,200 |
~ 9,000 |
~ 3,072 |
|
|
Catalog #
1 plate/pack 5 plates/pack Starter Kit* |
34411 34415 34450 |
34421 34425 34460 |
34811 34815 34850 |
34821 34825 34860 |
200-0563 200-0570 |
|
Here are some helpful tips when using AggreWell™ plates:
- Prior to using AggreWell™ plates, Anti-Adherence Rinsing Solution (Catalog #07010) is required for all cell types during plate preparation steps to ensure optimal performance. Anti-Adherence Rinsing Solution prevents cell adhesion and promotes efficient EB and spheroid formation. Add Anti-Adherence Rinsing Solution to each well to be used and centrifuge plate at 2000 x g (or at maximum speed) for 5 minutes in a swinging bucket rotor fitted with plate holders.
- To control just the EB or spheroid size, adjust the input cell seeding density.
- Use the right kind of plates (e.g. AggreWell™400 vs AggreWell™800) for generating the appropriate size EBs. Do not overload the wells to generate larger EBs as this will result in excess cells spilling over the microwell edges, leading to poor performance.
- When removing large EBs (2000 cells/EB or more) from the wells, use a large bore pipette tip or cut the end of a 1 mL pipette tip with sterile scissors to make the bore larger. This will prevent the breaking up of formed EBs.
- AggreWell™ plates are intended for one-time use only. Regardless of whether all the wells are used, or just one well is used, we do not recommend using the plate again to prevent contamination.
For more information, please contact Product and Scientific Support at techsupport@stemcell.com.
Request Pricing
Thank you for your interest in this product. Please provide us with your contact information and your local representative will contact you with a customized quote. Where appropriate, they can also assist you with a(n):
Estimated delivery time for your area
Product sample or exclusive offer
In-lab demonstration
By submitting this form, you are providing your consent to ƽ Technologies Canada Inc. and its subsidiaries and affiliates (“ƽ”) to collect and use your information, and send you newsletters and emails in accordance with our privacy policy. Please contact us with any questions that you may have. You can unsubscribe or change your email preferences at any time.
