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AggreWell™ HT

High-throughput 96-well microwell culture plates for easy and reproducible production of embryoid bodies and spheroids

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AggreWell™ HT

High-throughput 96-well microwell culture plates for easy and reproducible production of embryoid bodies and spheroids

Catalog #
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High-throughput 96-well microwell culture plates for easy and reproducible production of embryoid bodies and spheroids
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Product Advantages



  • Streamline high-throughput workflows with 900 ÎĽm microwells capable of forming 32 aggregates per well, ideal for scaling out


  • Ensure reproducibility with consistent and uniform-size cell aggregates


  • Enhance visualization with the meniscus-reducing feature for superior brightfield images


  • Easily form aggregates with as few as 50 cells per microwell


  • Transition seamlessly with compatibility across the AggreWell™ formats


What's Included

  • AggreWell™HT 96-well plate
    • 1 Unit (Catalog #200-0563)
    • 5 Units (Catalog #200-0570)
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. Anti-Adherence Rinsing Solution
    Anti-Adherence Rinsing Solution

    Rinsing solution for cultureware to prevent cell adhesion

Overview

Streamline your 3D culture workflows with AggreWell™HT, a high-throughput 96-well microwell plate designed to optimize the formation of uniform cell aggregates. Whether you’re generating spheroids or embryoid bodies (EBs), AggreWell™HT allows you to create up to 32 aggregates per well—significantly more than traditional ultra-low attachment plates—giving you higher statistical power, less variability, and more reliable results.

Ideal for experiments with rare cell types or limited samples, AggreWell™HT requires as few as 50 cells per microwell, ensuring you can maximize the use of your samples. The plate is also compatible with robotic liquid handling systems, and its meniscus-reducing feature improves the quality and ease of brightfield imaging, further enhancing your workflow efficiency. Note: Anti-Adherence Rinsing Solution (Catalog #07010) is required for optimal EB and spheroid formation.

AggreWell™HT is compatible with applications developed in both ´ˇ˛µ˛µ°ů±đ°Â±đ±ô±ô™400 and ´ˇ˛µ˛µ°ů±đ°Â±đ±ô±ô™800 formats, allowing for efficient scale-up of workflows. Learn more about how to use AggreWell™ or contact us for more information.
Subtype
Dishes and Plates
Application
Cell Culture, Characterization, Differentiation, Organoid Culture, Spheroid Culture, Toxicity Assay
Brand
AggreWell
Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Neuroscience, Organoids

Data Figures

AggreWell™HT features a meniscus-reducing feature and allows for the formation of aggregates from various initial seeding densities.

Figure 1. AggreWell™HT Promotes Cell Aggregation Across 32 Individual Microwells

(A) AggreWell™HT single-well cross section displaying the microwell array, featuring 32 microwells, and the meniscus-reducing step feature. Visualization of human embryonic stem cell (hESC) aggregates formed in AggreWell™HT after 72 hours from an initial seeding density of (B) 1,000 cells per microwell and (C) 10,000 cells per microwell.

AggreWell™HT promotes neural induction of embryonic stem cells to the same extent as ´ˇ˛µ˛µ°ů±đ°Â±đ±ô±ô™800.

Figure 2. AggreWell™HT Promotes Neural Induction in Embryoid Bodies Comparable to ´ˇ˛µ˛µ°ů±đ°Â±đ±ô±ô™800

Human embryonic stem (ES) cell (H9) aggregates cultured in (A) AggreWell™HT and (B) ´ˇ˛µ˛µ°ů±đ°Â±đ±ô±ô™800 microwell plates 24 hours post-seeding; scale bar = 500 ÎĽm. Morphology of Day 6 neural progenitor cell rosettes differentiated using an embryoid body (EB)-based protocol with Neural Induction Medium (Catalog #05835) in (C) AggreWell™HT and (D) ´ˇ˛µ˛µ°ů±đ°Â±đ±ô±ô™800 microwell plates; scale bar = 500 ÎĽm. (E) Neural marker expression of PAX6 and SOX1 is equivalent in both AggreWell™ plates at Day 18 following neural induction of H9 ES cells in both AggreWell™HT and ´ˇ˛µ˛µ°ů±đ°Â±đ±ô±ô™800 plates, as determined by flow cytometry (n = 4). Error bars = SD.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Name
Catalog #
200-0563
Lot #
All
Language
English
Document Type
Product Name
Catalog #
200-0570
Lot #
All
Language
English

Resources and Publications

Educational Materials (8)

Brochure
Brochure
How to Generate Uniform, Size-Controlled 3D Spheroid Cultures with AggreWell™ Microwell Plates
0:52
Video
How to Generate Uniform, Size-Controlled 3D Spheroid Cultures with AggreWell™ Microwell Plates
AggreWell™ - A New Technology for Uniform Embryoid Bodies
24:10
Webinar
AggreWell™ - A New Technology for Uniform Embryoid Bodies
Scientific Poster
Scientific Poster
Scientific Poster
Scientific Poster

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