Hybridoma and Mammalian Cell Line Development
Hybridomas, CHO cells and other mammalian cell lines are used for recombinant antibody and protein production. Generating stable cell lines which correctly and productively express the protein of interest is a lengthy and complex process. One of the most important considerations is achieving monoclonality of the cell line, while also encouraging robust cell expansion.
We've created these resources to help you accelerate your cell line development and get to production sooner.
Semi-Solid Cloning with ClonaCell™
Cloning hybridomas and CHO cells can be done faster and more efficiently using semi-solid cloning, compared to traditional cloning techniques. During semi-solid cloning, individual cells are immobilized within a viscous, semi-solid medium and grow into discrete, monoclonal colonies. Picking these colonies enables isolation of a large number of diverse clones with a high probability of monoclonality.
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ClonaCellâ„¢-HY ProcedureSelection and growth of mouse hybridomas to generate mouse monoclonal antibodies using ClonaCellâ„¢-HY including preparation of myeloma cells and splenocytes, fusion, selection and cloning, and harvesting -
Semi-Solid Cloning of CHO Cells Using ClonaCellâ„¢-CHO MediumProcedure for semi-solid cloning, including preparation of the cells, plating, colony picking, and expansion -
Considerations for Immunizing Mice for Hybridoma GenerationTechnical tip from our dedicated team of Product and Scientific Support specialists -
How to Perform Semi-Solid Cloning of CHO Cell Lines in 96-Well Plates Using ClonaCellâ„¢-CHO MediumThis video outlines the ClonaCellâ„¢-CHO 96-well plate semi-solid cloning protocol, including plating, liquid overlay, screening, and colony picking -
Biological Activity of Cytokines: Specific Activity (units/mg) vs. International Units (IU)Technical tip from our dedicated team of Product and Scientific Support specialists -
Mammalian Cell Cloning Procedure: Customizable Semi-Solid Cloning with ClonaCellâ„¢ FLEX MediumThis video outlines the ClonaCellâ„¢ FLEX procedure for semi-solid cloning, including preparation of the cells, plating, colony picking, and expansion
