Mammary Research
The mammary gland is a dynamic organ that undergoes extensive morphological changes through development, puberty, pregnancy, lactation and involution. Maintenance of the mammary epithelium during all of these stages is via mammary stem/progenitor cells.
Below is a collection of resources to support your mammary research.
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Mammary Stem Cells and Breast Cancer
This mini-review describes the organization of mammary epithelium, identification of mammary stem and progenitor cells, phenotypic profiling and cell culture methods for mammary epithelial cells, as well as the current understanding of mammary stem cells in the context of breast cancer.
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How to Prepare Conditioned Medium for the Expansion of Epithelial Cells<meta property="og:title" content="How to Prepare Conditioned Medium for Expansion of Epithelial Cells"/> <meta property="og:description" content="Learn how to generate conditioned medium from 3T3-J2 Irradiated Feeder Cells and Conditional Reprogramming Medium."/> <meta property="og:image" content="https://cdn.stemcell.com/media/images/social/propagenix-cm-protocol.png"/> <meta name="twitter:card" content="summary_large_image"/> <meta name="twitter:site" content="@º£½ÇÆƽâ°æTech"/> <meta name="twitter:image" content="https://cdn.stemcell.com/media/images/social/propagenix-cm-protocol.png"/> -
Optimization of ALDEFLUORâ„¢ ProtocolLearn how º£½ÇÆƽâ°æ Technologies' scientists doubled the signal strength in mammary epithelial cells with a few simple protocol modifications using ALDEFLUORâ„¢ to detect Aldehyde Dehydrogenase bright (ALDHbr) cells -
Culture Mammospheres and Tumorspheres with the MammoCultâ„¢ MediumVideo discusses MammoCultâ„¢, a medium that supports the growth of primary human mammary epithelial cells as well as numerous breast cancer cell lines -
Flow Cytometry Analysis of ALDH Bright Cells with the ALDEFLUORâ„¢ Assay KitThe ALDEFLUORâ„¢ fluorescent reagent system has supported over 200 publications to date -
Mammary Stem Cells and Breast CancerThe mammary gland is composed of a series of branched epithelial ducts that drain alveolar structures during lactation, with the epithelium embedded within a fibrous and fatty stroma -
How to Count 3D Sphere Cultures (Mammospheres, Neurospheres, Cancer Spheroids, and PSC Aggregates)Technique for counting 3D sphere cultures, such as mammospheres, neurospheres, or cancer spheroids
