Intestinal Organoids
The intestinal epithelium is a rapidly renewing tissue that can undergo complete cellular replacement every four to five days. These regenerative properties make the intestinal epithelium an attractive system for researchers studying epithelial regeneration, adult stem cell biology, disease modelling and cancer biology, both within and outside the context of the intestine.
Explore the resources below for information to support your intestinal research and the growth of intestinal organoids.
Featured
E-Book: Proven Protocols for Intestinal Organoid Culture
From getting started with intestinal organoids to advanced assays, this e-book provides curated protocols for the isolation and culture of human-derived intestinal organoids. Find proven protocols for a wide variety of well-established assays from immunocytochemical staining, toxicity testing, genetic manipulation, and monolayer differentiation.
Download Now >Key Applications of Intestinal Organoids
Crypt-Derived Intestinal Organoids
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Ootani A et al. (2009) Sustained in vitro intestinal epithelial culture within a Wnt-dependent stem cell niche. Nat Med 15(6): 701鈥6.
Sato T et al. (2009) Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche. Nature 459(7244): 262鈥5.
Sato T et al. (2011) Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett鈥檚 epithelium. Gastroenterology 141(5): 1762鈥72.
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Ootani A et al. (2009) Sustained in vitro intestinal epithelial culture within a Wnt-dependent stem cell niche. Nat Med 15(6): 701鈥6.
Sato T et al. (2009) Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche. Nature 459(7244): 262鈥5.
Sato T et al. (2011) Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett鈥檚 epithelium. Gastroenterology 141(5): 1762鈥72.
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PSC-Derived Intestinal Organoids
Spence JR et al. (2011) Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro. Nature 470(7332): 105鈥9.
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M煤nera JO et al. (2017) Methods Mol Biol 1597: 167鈥177.
Intestinal Cell Biology
de Jong PR et al. (2016) ERK5 signaling rescues intestinal epithelial turnover and tumour cell proliferation upon ERK1/2 abrogation. Nat Commun 7: 11551.
Dotti I et al. (2016) Alterations in the epithelial stem cell compartment could contribute to permanent changes in the mucosa of patients with ulcerative colitis. Gut. Epub ahead of print, DOI: 10.1136/gutjnl-2016-312609.
Igarashi M & Guarente L. (2016) mTORC1 and SIRT1 cooperate to foster expansion of gut adult stem cells during calorie restriction. Cell 166(2): 436鈥50.
Oudhoff MJ et al. (2016) SETD7 controls intestinal regeneration and tumorigenesis by regulating Wnt/尾-catenin and Hippo/YAP signaling. Dev Cell 37(1): 47鈥57.
Sasaki N et al. (2016) Reg4+ deep crypt secretory cells function as epithelial niche for Lgr5+ stem cells in colon. Proc Natl Acad Sci U S A 113(37): E5399-407.
Schewe M et al. (2016) Secreted phospholipases A2 are intestinal stem cell niche factors with distinct roles in homeostasis, inflammation, and cancer. Cell Stem Cell 19(1): 38鈥51.
Workman MJ et al. (2017) Nat Med 23(1): 49鈥59.
Zietek T et al. (2015) Intestinal organoids for assessing nutrient transport, sensing and incretin secretion. Sci Rep 5: 16831.
Mohanan V et al. (2018) Science 359(6380): 1161鈥1166.
Wang B et al. (2018) Cell Stem Cell 22(2): 206鈥220.
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Haber AL et al. (2017) Nature 551(7680): 333鈥339.
Kraiczy J et al. (2017) Gut.
Zhou W et al. (2018) J Cell Physiol 233(4): 3465鈥3475.
Hahn S et al. (2017) Sci Rep 7(1): 2435.
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Morgenstern Y et al. (2017) Embo J 36(20): 3046鈥3061.
Kraft CL et al. (2017) Oncotarget 8(61): 102923鈥102933.
Dotti I et al. (2016) Alterations in the epithelial stem cell compartment could contribute to permanent changes in the mucosa of patients with ulcerative colitis. Gut. Epub ahead of print, DOI: 10.1136/gutjnl-2016-312609.
Igarashi M & Guarente L. (2016) mTORC1 and SIRT1 cooperate to foster expansion of gut adult stem cells during calorie restriction. Cell 166(2): 436鈥50.
Oudhoff MJ et al. (2016) SETD7 controls intestinal regeneration and tumorigenesis by regulating Wnt/尾-catenin and Hippo/YAP signaling. Dev Cell 37(1): 47鈥57.
Sasaki N et al. (2016) Reg4+ deep crypt secretory cells function as epithelial niche for Lgr5+ stem cells in colon. Proc Natl Acad Sci U S A 113(37): E5399-407.
Schewe M et al. (2016) Secreted phospholipases A2 are intestinal stem cell niche factors with distinct roles in homeostasis, inflammation, and cancer. Cell Stem Cell 19(1): 38鈥51.
Workman MJ et al. (2017) Nat Med 23(1): 49鈥59.
Zietek T et al. (2015) Intestinal organoids for assessing nutrient transport, sensing and incretin secretion. Sci Rep 5: 16831.
Mohanan V et al. (2018) Science 359(6380): 1161鈥1166.
Wang B et al. (2018) Cell Stem Cell 22(2): 206鈥220.
Wichert R et al. (2017) Cell Rep 21(8): 2090鈥2103.
Haber AL et al. (2017) Nature 551(7680): 333鈥339.
Kraiczy J et al. (2017) Gut.
Zhou W et al. (2018) J Cell Physiol 233(4): 3465鈥3475.
Hahn S et al. (2017) Sci Rep 7(1): 2435.
Rong S et al. (2017) J Lipid Res 58(10): 1988鈥1998.
Morgenstern Y et al. (2017) Embo J 36(20): 3046鈥3061.
Kraft CL et al. (2017) Oncotarget 8(61): 102923鈥102933.
Epithelial Cell Biology
Blokzijl F et al. (2016) Tissue-specific mutation accumulation in human adult stem cells during life. Nature 538(7624): 260鈥4.
Kikuchi I et al. (2016) Nat Commun 7: 12887.
Nakajo A et al. (2016) J Cell Biol 212(3): 297鈥306.
Saxena A et al. (2017) Am J Physiol Gastrointest Liver Physiol 313(1): G26鈥揋38.
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Kikuchi I et al. (2016) Nat Commun 7: 12887.
Nakajo A et al. (2016) J Cell Biol 212(3): 297鈥306.
Saxena A et al. (2017) Am J Physiol Gastrointest Liver Physiol 313(1): G26鈥揋38.
Okkelman IA et al. (2016) PLOS One 11(12): E0167385.
Cancer Research
Borovski T et al. (2017) Gut 66(6): 1106鈥1115.
Fern谩ndez-Majada V et al. (2016) Nat Commun 7: 12508.
Fujii M et al. (2016) Cell Stem Cell 18(6): 827鈥38.
Liu W et al. (2016) Nat Commun 7: 10916.
Yan HHN et al. (2017) Gut 66(9): 1645鈥1656.
Yum MK et al. (2016) Cancer Res 76(15): 4559鈥68.
Duhachek-Muggy S et al. (2017) Radiat Res 188(5): 469鈥475.
Karki R et al. (2016) Nature 540: 583鈥587.
Dame MK et al. (2018) Development 145(6).
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Novellasdemunt L et al. (2017) Cell Rep 21(3): 612鈥627.
Banerjee A et al. (2016) Oncotarget 7(27): 41432鈥41444.
Liu W et al. (2016) Oncogene 35(40): 5237鈥5247.
Fern谩ndez-Majada V et al. (2016) Nat Commun 7: 12508.
Fujii M et al. (2016) Cell Stem Cell 18(6): 827鈥38.
Liu W et al. (2016) Nat Commun 7: 10916.
Yan HHN et al. (2017) Gut 66(9): 1645鈥1656.
Yum MK et al. (2016) Cancer Res 76(15): 4559鈥68.
Duhachek-Muggy S et al. (2017) Radiat Res 188(5): 469鈥475.
Karki R et al. (2016) Nature 540: 583鈥587.
Dame MK et al. (2018) Development 145(6).
Tauriello DVF et al. (2018) Nature 554(7693): 538鈥543.
Roper J et al. (2018) Nat Protoc 13(2): 217鈥234.
Fumagalli A et al. (2018) Nat Protoc 13(2): 235鈥247.
Regan JL et al. (2017) Cell Rep 21(10): 2813鈥2828.
Novellasdemunt L et al. (2017) Cell Rep 21(3): 612鈥627.
Banerjee A et al. (2016) Oncotarget 7(27): 41432鈥41444.
Liu W et al. (2016) Oncogene 35(40): 5237鈥5247.
Precision Medicine Screening
Vlachogiannis G et al. (2018) Science 359(6378): 920鈥926.
Beekman JM. (2016) Pediatr Pulmonol 51(S44): S23鈥揝34.
Hagemeijer MC et al. (2018) J Cyst Fibros 17(2s): S46鈥揝51.
Beekman JM. (2016) Pediatr Pulmonol 51(S44): S23鈥揝34.
Hagemeijer MC et al. (2018) J Cyst Fibros 17(2s): S46鈥揝51.
Therapeutic Development
Francies HE et al. (2016) Methods Mol Biol.
Zomer-van Ommen DD et al. (2016) J Med Chem 59(14): 6968鈥72.
Schulte ML et al. (2018) Nat Med 24(2): 194鈥202.
Arora K et al. (2017) JCI Insight 2(19).
Vijftigschild LAW et al. (2016) Eur Respir J 48(3): 768鈥79.
Zomer-van Ommen DD et al. (2016) J Med Chem 59(14): 6968鈥72.
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Arora K et al. (2017) JCI Insight 2(19).
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Disease Modeling
Dekkers JF et al. (2016) Eur Respir J 48(2): 451鈥8.
Schwank G et al. (2013) Cell Stem Cell 13(6): 653鈥8.
Huang K et al. (2018) Pediatr Res 83(5): 1031鈥1040.
Suzuki K et al. (2018) J Gastroenterol.
Dilly AK et al. (2017) Oncotarget 8(63): 106888鈥106900.
Xu P et al. (2017) Gut.
Donaldson DS et al. (2016) PLOS Pathog 12(12): E1006075.
Pattison AM et al. (2016) Infect Immun 84(10): 3083鈥91.
Schwank G et al. (2013) Cell Stem Cell 13(6): 653鈥8.
Huang K et al. (2018) Pediatr Res 83(5): 1031鈥1040.
Suzuki K et al. (2018) J Gastroenterol.
Dilly AK et al. (2017) Oncotarget 8(63): 106888鈥106900.
Xu P et al. (2017) Gut.
Donaldson DS et al. (2016) PLOS Pathog 12(12): E1006075.
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Intestinal Immunity and Inflammation
Gerbe F et al. (2016) Nature 529(7585): 226鈥30.
Ibiza S et al. (2016) Nature 535(7612): 440鈥443.
Lindemans CA et al. (2015) Nature 528(7583): 560鈥564.
Oancea I et al. (2017) Gut 66(1): 59鈥69.
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Howell KJ et al. (2018) Gastroenterology 154(3): 585鈥598.
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Aladegbami B et al. (2017) Sci Rep 7(1): 5580.
Bhushal S et al. (2017) Front Immunol 8: 671.
Sharma D et al. (2018) Gastroenterology 154(4): 948鈥964.
Ibiza S et al. (2016) Nature 535(7612): 440鈥443.
Lindemans CA et al. (2015) Nature 528(7583): 560鈥564.
Oancea I et al. (2017) Gut 66(1): 59鈥69.
Peuker K et al. (2016) Nat Med 22(5): 506鈥15.
Wang Y et al. (2017) Mucosal Immunol 10(2): 373鈥384.
Wilson SS et al. (2015) Mucosal Immunol 8(2): 352鈥61.
Van Opdenbosch N et al. (2017) Cell Rep 21(12): 3427鈥3444.
Howell KJ et al. (2018) Gastroenterology 154(3): 585鈥598.
Schwerd T et al. (2018) Mucosal Immunol 11(2): 562鈥574.
Aladegbami B et al. (2017) Sci Rep 7(1): 5580.
Bhushal S et al. (2017) Front Immunol 8: 671.
Sharma D et al. (2018) Gastroenterology 154(4): 948鈥964.
Microbiomics and Microbial Pathogenesis
Bartfeld S. (2016) Dev Biol 420(2): 262鈥270.
In Jg et al. (2016) Nat Rev Gastroenterol Hepatol 13(11): 633鈥642.
Tao L et al. (2016) Nature 538(7625): 350鈥355.
Wang J et al. (2018) Gastroenterology 154(6): 1737鈥1750.
Leber A et al. (2018) Front Immunol 9: 363.
Burger E et al. (2018) Cell Host Microbe 23(2): 177鈥190.
Zhang D et al. (2017) Sci Rep 7(1): 12621.
Yahiro K et al. (2018) Cell Death Discov 4: 22.
Entwistle LJ et al. (2017) Cell Host Microbe 22(4): 484鈥493.
Dhawan S et al. (2016) Am J Physiol Gastrointest Liver Physiol 311(5): G920鈥揋933.
Wang Y et al. (2017) Science 357(6354): 912鈥916.
In Jg et al. (2016) Nat Rev Gastroenterol Hepatol 13(11): 633鈥642.
Tao L et al. (2016) Nature 538(7625): 350鈥355.
Wang J et al. (2018) Gastroenterology 154(6): 1737鈥1750.
Leber A et al. (2018) Front Immunol 9: 363.
Burger E et al. (2018) Cell Host Microbe 23(2): 177鈥190.
Zhang D et al. (2017) Sci Rep 7(1): 12621.
Yahiro K et al. (2018) Cell Death Discov 4: 22.
Entwistle LJ et al. (2017) Cell Host Microbe 22(4): 484鈥493.
Dhawan S et al. (2016) Am J Physiol Gastrointest Liver Physiol 311(5): G920鈥揋933.
Wang Y et al. (2017) Science 357(6354): 912鈥916.
