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EasySep? Human CD56 Positive Selection Kit II

Immunomagnetic positive selection of human CD56+ cells

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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

EasySep? Human CD56 Positive Selection Kit II

Immunomagnetic positive selection of human CD56+ cells

Catalog #
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Immunomagnetic positive selection of human CD56+ cells
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Product Advantages


  • Fast and easy-to-use

  • Up to 98% purity

  • No columns required

What's Included

  • EasySep? Human CD56 Positive Selection Kit II (Catalog #17855)
    • EasySep? Human CD56 Positive Selection Cocktail II, 1 mL
    • EasySep? Dextran RapidSpheres? 50100, 1 mL
  • RoboSep? Human CD56 Positive Selection Kit II (Catalog #17855RF)
    • EasySep? Human CD56 Positive Selection Cocktail II, 1 mL
    • EasySep? Dextran RapidSpheres? 50100, 1 mL
    • RoboSep? Buffer (Catalog #20104)
    • RoboSep? Filter Tips (Catalog #20125)
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. EasySep™ Magnet
    EasySep? Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep? Buffer

    Cell separation buffer

Overview

Isolate highly purified human CD56+ cells from fresh or previously frozen peripheral blood mononuclear cells and human skeletal muscle (myoblasts and fibroblasts) culture samples by immunomagnetic positive selection, with the EasySep? Human CD56 Positive Selection Kit II. Widely used in published research for more than 20 years, EasySep? combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep? positive selection procedure, desired cells are labeled with antibody complexes recognizing CD56 and magnetic particles. The cocktail in this kit also contains an antibody to human Fc receptor to prevent non-specific binding. Labeled cells are separated using an EasySep? magnet and by simply pouring or pipetting off the unwanted cells. The cells of interest remain in the tube. Following magnetic cell isolation, the desired CD56+ cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction. The CD56 antigen is expressed on NK cells, NKT cells, and human myoblasts.

This product replaces the EasySep? Human CD56 Positive Selection Kit (Catalog #18055) for even faster cell isolations.

Learn more about how immunomagnetic EasySep? technology works or how to fully automate immunomagnetic cell isolation with RoboSep?. Alternatively, choose ready-to-use, ethically sourced, primary Human Peripheral Blood CD56+ Cells, Frozen isolated with EasySep? Human CD56 Positive Selection Kit II. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.

Magnet Compatibility
? EasySep? Magnet (Catalog #18000)
? “The Big Easy” EasySep? Magnet (Catalog #18001)
? EasyPlate? EasySep? Magnet (Catalog #18102)
? EasyEights? EasySep? Magnet (Catalog #18103)
? RoboSep?-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
NK Cells
Species
Human
Sample Source
Other, PBMC
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Chimerism, Immunology

Data Figures

Typical EasySep™ Human CD56 Positive Selection Profile

Figure 1. Typical EasySep™ Human CD56 Positive Selection Kit II (Catalog #17855)

Starting with human PBMCs, the CD56+ cell content of the isolated fraction is typically 96.3 ± 2.4% (mean ± SD), using the purple EasySep™ Magnet. In the above example, the purities of the start and final isolated fractions are 8.0% and 98.5%, respectively.

FACS Data for Anti-Human CD56 Antibody, Clone HCD56, Alexa Fluor® 488-Conjugated

Figure 2. FACS Data for Anti-Human CD56 Antibody, Clone HCD56, Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD56 Antibody, Clone HCD56, Alexa Fluor® (Catalog #60021AD) and Anti-Human CD45 Antibody, Clone HI30, APC (Catalog #60018AZ).

(B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD56 Positive Selection Kit (Catalog #17885) and labeled with Anti-Human CD56 Clone HCD56, Alexa Fluor® 488. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa Alexa Fluor® 488 isotype control antibody is shown (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Name
Catalog #
17855
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17855
Lot #
1000087747 or higher
Language
English
Document Type
Product Name
Catalog #
17855RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17855RF
Lot #
1000087747 or higher
Language
English
Document Type
Product Name
Catalog #
17855
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17855
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17855RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17855RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17855RF
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (12)

Brochure
Brochure
Brochure
Brochure
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
Human Immune Cytokines
Wallchart
Human Immune Cytokines
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll? or Lymphoprep?)
1:37
Video
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll? or Lymphoprep...
EasySep?のご説明 - 迅速かつ簡便な免疫磁気細胞分離テクノロジー
1:57
Video
EasySep?のご説明 - 迅速かつ簡便な免疫磁気細胞分離テクノロジー
How EasySep? Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep? Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Isolate Cells with a Simple Pour-Off: EasySep? Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep? Cell Separation Technology
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights? EasySep? Magnet
0:57
Video
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights? EasySep? Magnet
Cell Separation Solutions for HLA and Chimerism Analysis
26:43
Webinar
Cell Separation Solutions for HLA and Chimerism Analysis

Frequently Asked Questions

Can EasySep™ be used for either positive or negative selection?

Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).

How does the separation work?

Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Can EasySep™ be used to isolate rare cells?

Yes. We recommend a cell concentration of 2x108 cells/mL and a minimum working volume of 100 µL. Samples containing 2x107 cells or fewer should be suspended in 100 µL of buffer.

Are the EasySep™ magnetic particles FACS-compatible?

Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.

Can the EasySep™ magnetic particles be removed after enrichment?

No, but due to the small size of these particles, they will not interfere with downstream applications.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

For positive selection, can I perform more than 3 separations to increase purity?

Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

Publications (2)

PD-1 blockade potentiates HIV latency reversal ex vivo in CD4+ T cells from ART-suppressed individuals. R. Fromentin et al. Nature communications 2019 feb

Abstract

HIV persists in latently infected CD4+ T cells during antiretroviral therapy (ART). Immune checkpoint molecules, including PD-1, are preferentially expressed at the surface of persistently infected cells. However, whether PD-1 plays a functional role in HIV latency and reservoir persistence remains unknown. Using CD4+ T cells from HIV-infected individuals, we show that the engagement of PD-1 inhibits viral production at the transcriptional level and abrogates T-cell receptor (TCR)-induced HIV reactivation in latently infected cells. Conversely, PD-1 blockade with the monoclonal antibody pembrolizumab enhances HIV production in combination with the latency reversing agent bryostatin without increasing T cell activation. Our results suggest that the administration of immune checkpoint blockers to HIV-infected individuals on ART may facilitate latency disruption.
Myalgic encephalomyelitis/chronic fatigue syndrome patients exhibit altered T cell metabolism and cytokine associations. A. H. Mandarano et al. The Journal of clinical investigation 2019 dec

Abstract

Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a complex disease with no known cause or mechanism. There is an increasing appreciation for the role of immune and metabolic dysfunction in the disease. ME/CFS has historically presented in outbreaks, often has a flu-like onset, and results in inflammatory symptoms. Patients suffer from severe fatigue and post-exertional malaise. There is little known about the metabolism of specific immune cells in ME/CFS patients. To investigate immune metabolism in ME/CFS, we isolated CD4+ and CD8+ T cells from 53 ME/CFS patients and 45 healthy controls. We analyzed glycolysis and mitochondrial respiration in resting and activated T cells, along with markers related to cellular metabolism, and plasma cytokines. We found that ME/CFS CD8+ T cells have reduced mitochondrial membrane potential compared to healthy controls. Both CD4+ and CD8+ T cells from ME/CFS patients had reduced glycolysis at rest, while CD8+ T cells also had reduced glycolysis following activation. ME/CFS patients had significant correlations between measures of T cell metabolism and plasma cytokine abundance that differed from healthy control subjects. Our data indicate that patients have impaired T cell metabolism consistent with ongoing immune alterations in ME/CFS that may illuminate the mechanism behind this disease.
View All Publications

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