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EasySep? HLA Chimerism Whole Blood CD33 Positive Selection Kit

Immunomagnetic positive selection of human CD33+ myeloid cells

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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

EasySep? HLA Chimerism Whole Blood CD33 Positive Selection Kit

Immunomagnetic positive selection of human CD33+ myeloid cells

Catalog #
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Immunomagnetic positive selection of human CD33+ myeloid cells
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Product Advantages


  • Fast and easy-to-use

  • Up to 99% purity

  • No columns required

What's Included

  • EasySep? HLA Chimerism Whole Blood CD33 Positive Selection Kit (Catalog #17885)
    • EasySep? HLA Chimerism Whole Blood CD33 Positive Selection Cocktail, 3 x 1 mL
    • EasySep? Dextran RapidSpheres? 50101, 3 x 1 mL
    • EasySep? RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
  • RoboSep? HLA Chimerism Whole Blood CD33 Positive Selection Kit with Filter Tips (Catalog #17885RF)
    • EasySep? HLA Chimerism Whole Blood CD33 Positive Selection Cocktail, 3 x 1 mL
    • EasySep? Dextran RapidSpheres? 50101, 3 x 1 mL
    • EasySep? RBC Lysis Buffer 10X Concentrate, 10 mL
    • RoboSep? Buffer (Catalog #20104)
    • RoboSep? Filter Tips (Catalog #20125)
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. The Big Easy EasySep™ Magnet
    "The Big Easy" EasySep? Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep? Buffer

    Cell separation buffer

Overview

Isolate highly purified human CD33+ myeloid cells from fresh human whole blood or buffy coat samples by immunomagnetic positive selection, with the EasySep? HLA Chimerism Whole Blood CD33 Positive Selection Kit. Widely used in published research for more than 20 years, EasySep? combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep? positive selection procedure, desired cells are labeled with antibody complexes recognizing CD33 and magnetic particles. Labeled cells are separated using an EasySep? magnet and by simply pouring or pipetting off the unwanted cells. The cells of interest remain in the tube. Following magnetic cell isolation, the desired CD33+ cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction for lineage-specific chimerism analysis. The CD33 antigen is expressed on myeloid progenitor cells, granulocytes, and monocytes.

This product replaces the EasySep? Human Whole Blood CD33 Positive Selection Kit (Catalog #18287) and EasySep? HLA Whole Blood CD33 Positive Selection Kit (Catalog #18287HLA) for even faster cell isolations.

Learn more about how immunomagnetic EasySep? technology works or how to fully automate immunomagnetic cell isolation with RoboSep? to save time and increase laboratory throughput. Explore additional products optimized for your workflow, including those for cell characterization, cryopreservation, and more.

Magnet Compatibility
? “The Big Easy” EasySep? Magnet (Catalog #18001)
? EasyEights? EasySep? Magnet (Catalog #18103)
? RoboSep?-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
Granulocytes and Subsets, Myeloid Cells
Species
Human
Sample Source
Buffy Coat, Whole Blood
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep
Area of Interest
Chimerism, HLA, Immunology

Data Figures

Typical EasySep? HLA Chimerism Whole Blood CD33 Positive Selection Profile

Figure 1. Typical EasySep? HLA Chimerism Whole Blood CD33 Positive Selection Profile

Starting with fresh whole blood, the CD33+ cell content of the isolated fraction is typically 96 ± 2% (mean ± SD) using “The Big Easy” EasySep? Magnet. In the above example, the purities of the start (as assessed by labeling with anti-CD33) and the final isolated fractions are 68.0% and 96.2% (as assessed by labeling with anti-CD14 and anti-CD66b), respectively.
NOTE: RBCs were removed from the start sample by lysis prior to flow cytometry.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Name
Catalog #
17885RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17885
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17885RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17885RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17885RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17885RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17885
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17885
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17885
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (12)

Brochure
Brochure
Purity Assessment by Flow Cytometry for Lineage-Specific Chimerism Analysis
Technical Bulletin
Purity Assessment by Flow Cytometry for Lineage-Specific Chimerism Analysis
Fully Automated, High-Purity Cell Isolation for Chimerism Labs
Technical Bulletin
Fully Automated, High-Purity Cell Isolation for Chimerism Labs
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
Human Immune Cytokines
Wallchart
Human Immune Cytokines
How EasySep? Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep? Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
EasySep?のご説明 - 迅速かつ簡便な免疫磁気細胞分離テクノロジー
1:57
Video
EasySep?のご説明 - 迅速かつ簡便な免疫磁気細胞分離テクノロジー
Automate Cell Isolation with the RoboSep?-S Cell Separation Instrument
1:41
Video
Automate Cell Isolation with the RoboSep?-S Cell Separation Instrument
How to Prepare a Buffy Coat from Whole Blood
3:46
Video
How to Prepare a Buffy Coat from Whole Blood
Fully Automated Cell Separation for Chimerism Analysis with RoboSep?
56:02
Webinar
Fully Automated Cell Separation for Chimerism Analysis with RoboSep?
Scientific Poster

Publications (1)

Antibody-Mediated LILRB2-Receptor Antagonism Induces Human Myeloid-Derived Suppressor Cells to Kill Mycobacterium tuberculosis. V. K. Singh et al. Frontiers in immunology 2022

Abstract

Tuberculosis is a leading cause of death in mankind due to infectious agents, and Mycobacterium tuberculosis (Mtb) infects and survives in macrophages (M?¤s). Although M?¤s are a major niche, myeloid-derived suppressor cells (MDSCs) are an alternative site for pathogen persistence. Both M?¤s and MDSCs express varying levels of leukocyte immunoglobulin-like receptor B (LILRB), which regulate the myeloid cell suppressive function. Herein, we demonstrate that antagonism of LILRB2 by a monoclonal antibody (mab) induced a switch of human MDSCs towards an M1-macrophage phenotype, increasing the killing of intracellular Mtb. Mab-mediated antagonism of LILRB2 alone and its combination with a pharmacological blockade of SHP1/2 phosphatase increased proinflammatory cytokine responses and phosphorylation of ERK1/2, p38 MAPK, and NF-kB in Mtb-infected MDSCs. LILRB2 antagonism also upregulated anti-mycobacterial iNOS gene expression and an increase in both nitric oxide and reactive oxygen species synthesis. Because genes associated with the anti-mycobacterial function of M1-M?¤s were enhanced in MDSCs following mab treatment, we propose that LILRB2 antagonism reprograms MDSCs from an immunosuppressive state towards a pro-inflammatory phenotype that kills Mtb. LILRB2 is therefore a novel therapeutic target for eradicating Mtb in MDSCs.
View All Publications

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