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EasySep? HLA Chimerism Buffy Coat CD56 Positive Selection Kit

Immunomagnetic positive selection of human CD56+ cells from buffy coat

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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

EasySep? HLA Chimerism Buffy Coat CD56 Positive Selection Kit

Immunomagnetic positive selection of human CD56+ cells from buffy coat

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Immunomagnetic positive selection of human CD56+ cells from buffy coat
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Product Advantages


  • Fast and easy-to-use

  • Up to 99% purity

  • No columns required

What's Included

    • EasySep? HLA Chimerism Buffy Coat CD56 Positive Selection Kit (Catalog #17875)
        • EasySep? HLA Chimerism Buffy Coat CD56 Positive Selection Cocktail, 2 x 0.75 mL
        • EasySep? Dextran RapidSpheres, 2 x 1 mL
        • EasySep? RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
    • RoboSep? HLA Chimerism Buffy Coat CD56 Positive Selection Kit with Filter Tips (Catalog #17875RF)
        • EasySep? HLA Chimerism  Buffy Coat CD56 Positive Selection Cocktail, 2 x 0.75 mL
        • EasySep? Dextran RapidSpheres, 2 x 1 mL
        • EasySep? RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
        • RoboSep? Buffer (Catalog #20104) x 2
        • RoboSep? Filter Tips (Catalog #20125) x 2
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. The Big Easy EasySep™ Magnet
    "The Big Easy" EasySep? Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep? Buffer

    Cell separation buffer

Overview

Isolate highly purified human CD56+ cells from freshly prepared buffy coat and leukoreduction system chamber (LRSC; also known as an LRS cone) samples by immunomagnetic positive selection, with the EasySep? HLA Chimerism Buffy Coat CD56 Positive Selection Kit II. Widely used in published research for more than 20 years, EasySep? combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep? positive selection procedure, desired cells are labeled with antibody complexes recognizing CD56 and magnetic particles. Labeled cells are separated using an EasySep? magnet and by simply pouring or pipetting off the unwanted cells. The cells of interest remain in the tube. Following magnetic cell isolation, the desired CD56+ cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction for lineage-specific chimerism analysis. The CD56 antigen is expressed on NK cells and NKT cells.

This product replaces the EasySep? Human Buffy Coat CD56 Positive Selection Kit (Catalog #18085) and EasySep? HLA Buffy Coat CD56 Positive Selection Kit (Catalog #18085HLA), for even faster cell isolations.

Learn more about how immunomagnetic EasySep? technology works or how to fully automate immunomagnetic cell isolation with RoboSep? to save time and increase laboratory throughput. Explore additional products optimized for your workflow, including those for cell characterization, cryopreservation, and more.

Magnet Compatibility
? “The Big Easy” EasySep? Magnet (Catalog #18001)
? EasyEights? EasySep? Magnet (Catalog #18103)
? RoboSep?-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
NK Cells
Species
Human
Sample Source
Buffy Coat
Selection Method
Positive
Brand
EasySep
Area of Interest
Chimerism, HLA, Immunology

Data Figures

EasySep? HLA Chimerism Buffy Coat CD56 Positive Selection Profile

Figure 1. Typical EasySep? HLA Chimerism Buffy Coat CD56 Positive Selection Profile

Starting with freshly prepared buffy coat, the CD56+ cell content of the isolated fraction typically ranges from 95.8 - 99.5% (gated on CD45+ using “The Big Easy” EasySep? Magnet). In the above example, the purities of the start and final isolated fractions are 8.5% and 98.4%, respectively. NOTE: RBCs were removed from the start sample by lysis prior to flow cytometry.

FACS Data for Anti-Human CD56 Antibody, Clone HCD56, APC-Conjugated

Figure 2. FACS Data for Anti-Human CD56 Antibody, Clone HCD56, APC-Conjugated

Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep? HLA Buffy Coat CD56 Positive Selection Kit (Catalog #17875) and labeled with Anti-Human CD56 Antibody, Clone HCD56, APC (Catalog #60021AZ). Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa APC isotype control antibody is shown (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

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17875RF
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English
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17875RF
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English
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17875
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English
Document Type
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17875
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English
Document Type
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17875RF
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English
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17875RF
Lot #
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English
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17875RF
Lot #
All
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English
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Product Name
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17875RF
Lot #
All
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English
Document Type
Product Name
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17875
Lot #
All
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English
Document Type
Product Name
Catalog #
17875
Lot #
All
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English
Document Type
Product Name
Catalog #
17875
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (14)

Brochure
Brochure
Purity Assessment by Flow Cytometry for Lineage-Specific Chimerism Analysis
Technical Bulletin
Purity Assessment by Flow Cytometry for Lineage-Specific Chimerism Analysis
Fully Automated, High-Purity Cell Isolation for Chimerism Labs
Technical Bulletin
Fully Automated, High-Purity Cell Isolation for Chimerism Labs
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
Human Immune Cytokines
Wallchart
Human Immune Cytokines
How EasySep? Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep? Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
How to Prepare a Buffy Coat from Whole Blood
3:46
Video
How to Prepare a Buffy Coat from Whole Blood
How to Recover Cells from a Leukocyte Reduction System (LRS) Cone
4:55
Video
How to Recover Cells from a Leukocyte Reduction System (LRS) Cone
Automate Cell Isolation with the RoboSep?-S Cell Separation Instrument
1:41
Video
Automate Cell Isolation with the RoboSep?-S Cell Separation Instrument
EasySep?のご説明 - 迅速かつ簡便な免疫磁気細胞分離テクノロジー
1:57
Video
EasySep?のご説明 - 迅速かつ簡便な免疫磁気細胞分離テクノロジー
The Nuts and Bolts of Lineage Chimerism Testing
33:41
Webinar
The Nuts and Bolts of Lineage Chimerism Testing
Fully Automated Cell Separation for Chimerism Analysis with RoboSep?
56:02
Webinar
Fully Automated Cell Separation for Chimerism Analysis with RoboSep?
Scientific Poster

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