How to Choose Your Neural Organoid Model System
Human pluripotent stem cell (hPSC)-derived neural organoids offer unique opportunities to study the human brain using physiologically relevant and complex 3D models. Neural organoid differentiation strategies may be broadly classified into two major types of methodologies–patterned and unpatterned. Depending on the desired downstream application or readout, one (or both) of these options may provide a powerful tool to help you address exciting new research questions.
Patterned Neural Organoids
Patterned neural organoid strategies rely on directed differentiation using small molecule patterning factors to generate specific brain regions of interest.
Key Features & Applications:
- Highly reproducible and homogeneous structural morphology for greater study sensitivity
- Easily scalable with ´¡²µ²µ°ù±ð°Â±ð±ô±ôâ„¢ for high-throughput applications to generate hundreds of organoids in a single batch
- Ideal for:
- Studying inter-organoid interactions by generating assembloids1,2 (modular combinations of different patterned organoids into one system)
- Co-culturing with hPSC-derived microglia to model neuroinflammation
- High-throughput screening
For more information and protocol details, please refer to the product pages for STEMdiffâ„¢ Dorsal Forebrain Organoid Differentiation Kit and STEMdiffâ„¢ Ventral Forebrain Organoid Differentiation Kit.
Unpatterned Neural Organoids
Unpatterned neural organoid strategies allow differentiation to occur spontaneously, without the addition of specific patterning factors.
Key Features & Applications:
- Rich cellular and structural diversity obtained
- Multiple brain regions generated within a single organoid
- Amenable to culturing within Matrigel® droplets or in liquid Matrigel® conditions
- Ideal for:
- Studying intra-organoid interactions between brain regions
- Modeling spontaneous neurodevelopment3
- Using as a platform for generating new or modified organoid models (ie. choroid plexus4)
- Infectious disease research
For more information and protocol details, please refer to the product page for STEMdiffâ„¢ Cerebral Organoid Kit.
Still unsure which model is right for your research? We can help. Contact us by phone or email or use the LiveChat function on this page to discuss your specific needs and applications.
Related Resources
- Sloan SA et al. (2018) Nat Protoc 13(9): 2062–85.
- Andersen J et al. (2020) Cell 183(7): 1913–29.
- Lancaster MA et al. (2013) Nature 501(7467): 373–9.
- Pellegrini L et al. (2020) Science 369(6500): eaaz5626.
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