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RoboSepā„¢-C Human CD8+ T Cell Isolation Kit

Immunomagnetic negative selection kit

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RoboSepā„¢-C Human CD8+ T Cell Isolation Kit

Immunomagnetic negative selection kit

Catalog #
(Select a product)
Immunomagnetic negative selection kit
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Product Advantages


  • Fully automated large-scale isolation in a closed system

  • Up to 94% purity with high recovery

  • Untouched, viable cells

What's Included

  • RoboSepā„¢-C Human CD8+ T Cell Isolation Cocktail, 10 mL
  • RoboSepā„¢-C Dextran RapidSpheresā„¢ 30106, 10 mL
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. RoboSep™-C PBS/EDTA Buffer
    RoboSepā„¢-C PBS/EDTA Buffer

    Cell separation buffer

  2. RoboSep™-C Tubing Set
    RoboSepā„¢-C Tubing Set

    For automated cell isolation with RoboSepā„¢-C

Overview

The RoboSepā„¢-C Human CD8+ T Cell Isolation Kit is designed to isolate untouched and highly purified CD8+ T cells from fresh human peripheral blood leukopaks using RoboSepā„¢-C (Catalog #100-0185 and #100-0186) for automated immunomagnetic negative selection.

This kit targets non-CD8+ T cells for removal with antibodies recognizing specific cell surface markers. Using RoboSepā„¢-C in a fully automated protocol, unwanted cells are labeled with antibodies and magnetic particles, and separated without columns using a RoboSepā„¢-C magnet. The desired untouched cells are washed and concentrated by RoboSepā„¢-C and transferred to a final product bag. Isolated cells are immediately available for downstream applications such as flow cytometry, culture, or cryopreservation.
Magnet Compatibility
ā€¢ RoboSepā„¢-C (Catalog #100-0185)
ā€¢ RoboSepā„¢-C (Catalog #100-0186)
Subtype
Cell Isolation Kits
Cell Type
T Cells, T Cells, CD8+
Species
Human
Sample Source
Leukapheresis
Selection Method
Negative
Application
Cell Isolation
Brand
RoboSep
Area of Interest
Drug Discovery and Toxicity Testing, Immunology, Cell Therapy Development

Data Figures

FACS Profile Results with RoboSepā„¢-C Human CD8+ T Cell Isolation Kit

Figure 1. Typical RoboSepā„¢-C Human CD8+ T Cell Isolation Profile

Starting with a fresh human peripheral blood leukopak, the CD8+ T cell content (CD3+CD8+) of the isolated fraction is typically 86.8 Ā± 5.4% (mean Ā± SD). In the above example, the purities of the start and final isolated fractions are 16.5% and 93.3%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Name
Catalog #
100-0202
Lot #
All
Language
English
Document Type
Product Name
Catalog #
100-0202
Lot #
All
Language
English
Document Type
Product Name
Catalog #
100-0202
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (6)

Brochure
Brochure
Genome Editing of Human Primary T Cells Using CRISPR-Cas9
Technical Bulletin
Genome Editing of Human Primary T Cells Using CRISPR-Cas9
Production of Chimeric Antigen Receptor T Cells
Wallchart
Production of Chimeric Antigen Receptor T Cells
CAR T Cell Manufacturing Workflow: Isolation, Activation and Expansion
0:59
Video
CAR T Cell Manufacturing Workflow: Isolation, Activation and Expansion
Quick and Easy Isolation of T Cells and Other Immune Cells from Large-Volume Samples
37:09
Webinar
Quick and Easy Isolation of T Cells and Other Immune Cells from Large-Volume Samples

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Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT ŗ£½ĒĘĘ½ā°ę, REFER TO WWW.ŗ£½ĒĘĘ½ā°ę.COM/COMPLIANCE.

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