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Figure 1. Growth of Human Pluripotent Stem Cells (hPSCs) in �ձ𳧸�™-������ 3D

�ձ𳧸�™-������ 3D supports the expansion and high viability of hPSCs over multiple passages in aggregate suspension culture. Shown are (A) cumulative viable cells, (B) daily fold expansion, and (C) end-of-passage viabilities in human ES cell lines (H1 and H7) and human iPS cell lines (WLS-1C and STiPS-F016) over 5 passages. Error bars represent ± SD, n = 3.

Figure 2. hPSCs Cultured in �ձ𳧸�™-������ 3D Express Markers of the Undifferentiated State and Differentiate to the Three Germ Layers

Shown are (A) OCT-4 and TRA-1-60 expression and (B) trilineage differentiation potential (ectoderm: % Nestin+/Pax6+, mesoderm: % Brach+, and endoderm: % Sox-17+/CXCR4+) using the STEMdiff™ Trilineage Differentiation Kit for multiple hPSC lines after 5 passages in �ձ𳧸�™-������ 3D. Error bars represent ± SD, n = 3.

Figure 3. Metabolic Profile of hPSCs During Culture in �ձ𳧸�™-������ 3D

(A) Glucose concentration in �ձ𳧸�™-������ 3D is maintained at optimal levels throughout the culture period. Dotted line represents the glucose level in �ձ𳧸�™-������ 3D Complete Seed Medium. (B) Lactate concentration is maintained at low levels (below 15 mM as indicated by the dotted line) throughout the culture period for multiple hPSC lines after 5 passages in �ձ𳧸�™-������ 3D. Error bars represent ± SD, n = 3.

Figure 4. Morphology of hPSC Aggregates in �ձ𳧸�™-������ 3D

Multiple hPSC lines maintained in �ձ𳧸�™-������ 3D depict morphology characteristic of hPSC aggregates in suspension culture, namely a mostly spherical shape, clear edges (not smooth or shiny), even color, a dimpled or pockmarked appearance, and are less than 400 µm in size.