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STEMdiffâ„¢ Lung Progenitor Kit

Serum-free medium for efficient and reproducible generation of lung progenitor cells from human ES and iPS cells

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Need a high-quality cell source? Use the hiPSC SCTi003-A (female) control line, manufactured with mTeSRâ„¢ Plus.

STEMdiffâ„¢ Lung Progenitor Kit

Serum-free medium for efficient and reproducible generation of lung progenitor cells from human ES and iPS cells

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Serum-free medium for efficient and reproducible generation of lung progenitor cells from human ES and iPS cells
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Product Advantages


  • Differentiate hPSCs to lung progenitor cells with a robust medium, optimized to work with ³¾°Õ±ð³§¸éâ„¢1 or mTeSRâ„¢ Plus

  • Obtain lung progenitor cells capable of downstream differentiation to proximal and distal airway cells

  • Maximize experimental reproducibility with an optimized, serum-free formulation

  • Convenient format and easy-to-use protocol

What's Included

  • STEMdiffâ„¢ Endoderm Basal Medium, 100 mL
  • STEMdiffâ„¢ Definitive Endoderm Supplement MR (100X), 0.35 mL
  • STEMdiffâ„¢ Definitive Endoderm Supplement CJ (100X), 1.1 mL
  • STEMdiffâ„¢ Lung Basal Medium, 180 mL
  • STEMdiffâ„¢ Lung Supplement (10X), 20 mL
  • STEMdiffâ„¢ Lung Progenitor Supplement 1 (100X), 0.55 mL
  • STEMdiffâ„¢ Lung Progenitor Supplement 2 (100X), 1.45 mL
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. Gentle Cell Dissociation Reagent
    Gentle Cell Dissociation Reagent

    cGMP, enzyme-free cell dissociation reagent

  2. D-PBS (Without Ca++ and Mg++)
    D-PBS (Without Ca++ and Mg++)

    Dulbecco’s phosphate-buffered saline without calcium and magnesium

  3. Y-27632 (Dihydrochloride)
    Y-27632 (Dihydrochloride)

    RHO/ROCK pathway inhibitor; Inhibits ROCK1 and ROCK2

  4. mTeSR™1
    ³¾°Õ±ð³§¸éâ„¢1

    cGMP, feeder-free maintenance medium for human ES and iPS cells

Overview

STEMdiffâ„¢ Lung Progenitor Kit is a serum-free culture medium system for efficient and reproducible generation of lung progenitor cells from human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) through three stages of differentiation: 1) definitive endoderm, 2) anterior foregut endoderm, and 3) lung progenitor cells. Differentiated cells will express NKX2.1, a key marker of lung progenitor cells. The resulting cells can be further matured towards proximal or distal airway cells using published protocols for the study of lung diseases and lung development.
Subtype
Specialized Media
Cell Type
Airway Cells, Pluripotent Stem Cells
Species
Human
Application
Cell Culture, Differentiation, Organoid Culture
Brand
STEMdiff
Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Epithelial Cell Biology, Respiratory Research, Stem Cell Biology
Formulation Category
Serum-Free

Data Figures

Illustration showing the protocol, media, and three stages (definitive endoderm, anterior foregut endoderm, and lung progenitors) invoved in the differentiation of human PSCs into lung progenitor cells using the STEMdiffâ„¢ Lung Progenitor Kit.

Figure 1. Generation of Lung Progenitor Cells from hESCs/hiPSCs with the STEMdiffâ„¢ Lung Progenitor Kit

Human embryonic (hESCs) or induced (hiPSCs) pluripotent stem cell cultures progress through a simple three-stage process to generate lung progenitor cells. hESCs or hiPSCs clumps are first seeded in ³¾°Õ±ð³§¸éâ„¢1 or mTeSRâ„¢ Plus. On Day 1, differentiation is initiated with Medium DE-1 (STEMdiffâ„¢ Endoderm Basal Medium containing Supplement MR and Supplement CJ). Subsequently, on Days 2 and 3, the medium is changed to Medium DE-2 (STEMdiffâ„¢ Endoderm Basal Medium containing Supplement CJ) for definitive endoderm patterning. On Day 4, to initiate anterior foregut endoderm patterning, the endoderm monolayer is passaged in Medium LP-1 (STEMdiffâ„¢ Lung Basal Medium, Lung Supplement (10X), and Supplement 1) and Y-27632 (Dihydrochloride). Finally, at Day 7, the cells are differentiated into the lung progenitor stage with Medium LP-2 (STEMdiffâ„¢ Lung Basal Medium, Lung Supplement (10X), and Supplement 2).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

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100-0230
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English
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100-0230
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English
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100-0230
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English
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100-0230
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100-0230
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100-0230
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English
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100-0230
Lot #
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English
Document Type
Product Name
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100-0230
Lot #
All
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English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (13)

Brochure
Brochure
Brochure
Brochure
How to Prepare Single Cells from PSC-Derived Epithelial Cultures for Flow Cytometry Analysis
Protocol
How to Prepare Single Cells from PSC-Derived Epithelial Cultures for Flow Cytometry Analysis
Cellular Organization and Biology of the Respiratory System
Wallchart
Cellular Organization and Biology of the Respiratory System
Directed Differentiation of Pluripotent Stem Cells
Wallchart
Directed Differentiation of Pluripotent Stem Cells
Derivation and Applications of Human Pluripotent Stem Cells
Wallchart
Derivation and Applications of Human Pluripotent Stem Cells
Human Lung Development and Disease: Lessons from Organoids
26:16
Webinar
Human Lung Development and Disease: Lessons from Organoids
STEMdiffâ„¢ Kits for Robust and Efficient Differentiation of hPSCs to Multiple Cell Types
20:24
Webinar
STEMdiffâ„¢ Kits for Robust and Efficient Differentiation of hPSCs to Multiple Cell Types
hPSC-Derived Lung Models as Tools for Respiratory Research
17:17
Webinar
hPSC-Derived Lung Models as Tools for Respiratory Research
Making Lung Cells from Pluripotent Stem Cells: Disease Modeling and Future Therapies
44:40
Webinar
Making Lung Cells from Pluripotent Stem Cells: Disease Modeling and Future Therapies
Scientific Poster

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Need a high-quality cell source? Use the hiPSC SCTi003-A (female) control line, manufactured with mTeSRâ„¢ Plus.
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