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SARS-CoV-2 Recombinant Spike Protein, aa16-685 (HEK293-expressed)

SARS-CoV-2 recombinant spike protein, amino acids Val16-Arg685, FLAG & His tags

SARS-CoV-2 Recombinant Spike Protein, aa16-685 (HEK293-expressed)

SARS-CoV-2 recombinant spike protein, amino acids Val16-Arg685, FLAG & His tags

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SARS-CoV-2 recombinant spike protein, amino acids Val16-Arg685, FLAG & His tags
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Overview

SARS-CoV-2 Recombinant Spike Protein, aa16-685 is expressed in HEK293 cells and is one of four structural proteins encoded by the SARS-CoV-2 genome. The Spike Protein plays a key role in attachment to host cells, allowing invasion through clathrin-mediated endocytosis. The Spike Protein can be cleaved by host cell proteases after aa685 to yield the N-terminal S1 subunit and C-terminal S2 region. The S1 subunit is responsible for interacting with the host cell receptor (angiotensin-converting enzyme II) through a receptor-binding domain that is highly conserved with SARS-CoV. The S1 subunit has two conformations: a 鈥榙own鈥 conformation in which the receptor is inaccessible, and an 鈥榰p鈥 conformation in which the receptor is accessible. These conformational changes are key for monoclonal antibody drugs and vaccine development. SARS-CoV-2 Recombinant Spike Protein contains a polyhistidine tag at the amino terminus; it also contains a FLAG tag at the carboxy terminus.
Subtype
Recombinant Proteins
Alternative Names
S protein, Spike glycoprotein
Cell Type
B Cells, Lymphocytes, Plasma, T Cells, T Cells, CD4+, T Cells, CD8+
Species
Human
Area of Interest
Infectious Diseases
Purity
鈮 85%

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Name
Catalog #
100-0595, 100-0594
Lot #
All
Language
English
Document Type
Product Name
Catalog #
100-0595, 100-0594
Lot #
All
Language
English

Resources and Publications

Educational Materials (6)