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Streamline your assays with ready-to-use, ethically sourced, mobilized primary CD34+ cells. We help you get the cells you need with personalized service, custom products, flexible delivery times, and the option to reserve entire lots to prescreen cells for applications.
Donors are mobilized with:
鈥 G-CSF: a maximum of 10 碌g/kg/day of granulocyte colony-stimulating factor for 3 - 5 days prior to collection (Catalog #70060)
鈥 Plerixafor: a maximum of 0.24 mg/kg plerixafor for 1 day prior to collection (Catalog #70075)
鈥 G-CSF and Plerixafor: a maximum of 10 碌g/kg/day of G-CSF for 3 - 5 days prior to collection and a maximum of 0.24 mg/kg of plerixafor for 1 day prior to collection (Catalog #70073)
Isolated from leukapheresis samples using positive immunomagnetic separation techniques and cryopreserved in serum-free medium, cells are obtained using Institutional Review Board (IRB)-approved consent forms and protocols. Additional documentation and high-resolution HLA typing (Class I and Class II alleles and CMV status) are available upon request. Acid-citrate-dextrose solution A (ACDA) is added as an anticoagulant. Donor specifications (e.g. BMI category, smoking status, ethnicity, etc.) can be requested in the comment box above, after selecting from the product options. Donors are screened for HIV-1, HIV-2, hepatitis B, and hepatitis C.
Certain products are only available in select territories. Please contact your local sales representative or Product & Scientific Support at techsupport@stemcell.com for further information.
Figure 1. Cryopreserved G-CSF Mobilized Human Peripheral Blood CD34+ Cells Generate Hematopoietic Colonies in CFU Assays
Cryopreserved G-CSF Mobilized Human Peripheral Blood CD34+ Cells (Catalog #70060) were thawed, plated at a concentration of 5 x 10^2 viable cells/dish and cultured in MethoCult鈩 Optimum Medium (Catalog #04034) for 14 days. Shown are colonies produced by hematopoietic progenitor cells in the sample, imaged with STEMvision鈩.
Figure 2. StemSpan鈩 Media Supports Equal or Greater Expansion of Mobilized Peripheral Blood HSPCs Compared to Other Commercial Media
Purified CD34+ cells derived from G-CSF mobilized peripheral blood (mPB) were cultured at a concentration of 10000 cells per mL in StemSpan鈩 media (SFEM, SFEM II, AOF, and XF, orange bars), and in six media from other suppliers (Commercial Alternatives, grey bars). All media were supplemented with StemSpan鈩 CD34+ Expansion Supplement and UM729 (1 uM). After 7 days of culture, the (A) frequency and (B) cell expansion of viable CD34bright, CD34bright CD45RA-CD90鈦, and CD34bright CD45RA-CD90鈦篊D133鈦篍PCR鈦 cells were analyzed by flow cytometry, as described in Figure 1. Fold expansion was normalized to StemSpan鈩 SFEM (the number above the bar indicates average yield per input CD34+ cell 卤 SD). Compared to the competitor media tested, StemSpan鈩 media showed similar or higher expansion of CD34bright, CD34bright CD45RA-CD90+, and CD34bright CD45RA-CD90+CD133+EPCR+ cells (each subsequent cell subset being progressively more enriched for functional stem/progenitor cells). Data shown are mean 卤 SEM (n = 5).
This product is designed for use in the following research area(s) as part
of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we
offer to support each research area.
Mobilized Human Peripheral Blood CD34+ Cells, Frozen
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PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT 海角破解版, REFER TO WWW.海角破解版.COM/COMPLIANCE.
