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The Human Interleukin 17A (IL-17A) ELISA Kit is designed for the quantitative detection and measurement of human IL-17A in biological fluids such as serum, plasma, and cell culture supernatants. IL-17A is secreted by Th17 cells, CD8+ T cells, γδ T cells, natural killer T cells, B cells, innate lymphoid cells, and mesenchymal stromal cells. IL-17A is a pro-inflammatory cytokine that plays a key role in the defense against pathogens, activates the NF-kB, MAPK, and C/EBP pathways, and in conjunction with TNF-α, induces sustained neutrophil recruitment during inflammation.
The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the cytokine. The captured cytokine is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase (SA-HRP), which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate 3,3’,5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of cytokine in the sample. The concentration of the cytokine is determined by comparison to a serial dilution of the cytokine standard analyzed in parallel.
Subtype
Complete Kits
Cell Type
B Cells, Lymphocytes, Mesenchymal Stem and Progenitor Cells, T Cells, T Cells, CD4+, T Cells, CD8+, T Cells, Other Subsets
• Reportable Range: 3.2 - 1000 pg/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity.
• Sensitivity: The limit of detection of this assay is 1 pg/mL. This is the analyte concentration with absorbance two standard deviations higher than the zero standard.
• Accuracy: The analyte standard of this ELISA was calibrated against NIBSC international standard 01/420.
• Recovery: A mid-curve recovery of 96 - 119% was determined by spiking defined amounts of analyte standard into serum or plasma samples in repeated experiments.
• Precision: The intra-assay precision of this assay is 3.9% (CV). The inter-assay precision of this assay is 7.3% (CV).
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