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Figure 1. Typical EasySep™ Mouse CD138 Positive Cell Isolation Profile

(A) Starting with immunized BALB/c mouse splenocytes, the CD138+ cell content of the isolated fraction is typically 81.5 ¡À 4.9% (mean ¡À SD). In the above example, the purities of the start and final isolated fractions are 4.6% and 83.3%, respectively.

(B) Starting with immunized BALB/c mouse splenocytes, the plasma cell (CD138+CD267 (TACI)+) content is typically 68.5 ¡À 11.3% (mean ¡À SD). In the above example, the purities of the start and final isolated fractions are 1.3% and 70.2%, respectively.

(C) Starting with na?ve C57BL/6 mouse splenocytes, the CD138+ cell content of the isolated fraction is typically 78.3 ¡À 5.7% (mean ¡À SD). In the above example, the purities of the start and final isolated fractions are 5.0% and 79.5%, respectively.

(D) Starting with na?ve C57BL/6 mouse splenocytes, the plasma cell (CD138+CD267 (TACI)+) content is typically 50.8 ¡À 10.0 % (mean ¡À SD). In the above example, the purities of the start and final isolated fractions are 0.4% and 56.3%, respectively.

(E) Isolated CD138+ cells or total splenocytes from mice immunized with various antigens were fused with Sp2/0 mouse myeloma cells and plated in semi-solid medium using ClonaCell™-HY Hybridoma Kit (Catalog #03800). The % antigen-specific hit rate was determined by ELISA. The % antigen-specific hit rates for total splenocytes and CD138+ cells were 4.1 ¡À 1.6% and 28.8 ¡À 11.0% (mean ¡À SD), respectively.

(F) Isolated CD138+ cells or total splenocytes from mice immunized with various antigens were fused with Sp2/0 mouse myeloma cells and plated in semi-solid medium using ClonaCell™-HY Hybridoma Kit (Catalog #03800). The % antibody-secreting hybridomas was determined by ELISA. The % antibody-secreting hybridomas for total splenocytes and CD138+ cells were 33.0 ¡À 8.7% and 99.3 ¡À 0.6% (mean ¡À SD), respectively.