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EasySep? Human Eosinophil Isolation Kit

Immunomagnetic negative selection of untouched human eosinophils (CD16-CD66b+CD45+) from fresh human PMNCs

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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

EasySep? Human Eosinophil Isolation Kit

Immunomagnetic negative selection of untouched human eosinophils (CD16-CD66b+CD45+) from fresh human PMNCs

Catalog #
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Immunomagnetic negative selection of untouched human eosinophils (CD16-CD66b+CD45+) from fresh human PMNCs
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Product Advantages


  • Fast, easy-to-use and column-free

  • Up to 99% purity

  • Untouched, viable cells

What's Included

  • EasySep? Human Eosinophil Isolation Kit (Catalog #17956)
    • EasySep? Human Eosinophil Isolation Cocktail, 1 mL
    • EasySep? Dextran RapidSpheres?, 1 mL
  • RoboSep? Human Eosinophil Isolation Kit with Filter Tips (Catalog #17956RF)
    • EasySep? Human Eosinophil Isolation Cocktail, 1 mL
    • EasySep? Dextran RapidSpheres?, 1 mL
    • RoboSep? Buffer (Catalog #20104)
    • RoboSep? Filter Tips (Catalog #20125)
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. EasySep™ Magnet
    EasySep? Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep? Buffer

    Cell separation buffer

Overview

Easily and efficiently isolate highly purified human eosinophils (CD16-CD66b+CD45+) from fresh human peripheral blood polymorphonuclear cells (PMNCs) by immunomagnetic negative selection, with the EasySep? Direct Human Eosinophil Isolation Kit. Widely used in published research for more than 20 years, EasySep? combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep? negative selection procedure, unwanted cells are labeled with antibody complexes and magnetic particles. The magnetically labeled cells are then separated from the untouched desired eosinophils by using an EasySep? magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation, the desired eosinophils are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

Learn more about how immunomagnetic EasySep? technology works or how to fully automate immunomagnetic cell isolation with RoboSep?. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
Magnet Compatibility
? EasySep? Magnet (Catalog #18000)
? “The Big Easy” EasySep? Magnet (Catalog #18001)
? EasyEights? EasySep? Magnet (Catalog #18103)
? RoboSep?-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
Granulocytes and Subsets
Species
Human
Sample Source
PMNC, Whole Blood
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology

Data Figures

Figure 1. Typical EasySep? Human Eosinophil Isolation Profile

Starting with whole blood prepared using RBC lysis or HetaSep?, the eosinophil content (CD16-CD66b+CD45+) of the isolated fraction is typically 96.5 ± 2.5% (mean ± SD) using the purple EasySep? Magnet. In the above examples, the purities of the start and final isolated fractions prepared using RBC lysis are 1.8% and 97.8% (gated on CD45+), respectively, and when prepared using HetaSep?, the purities are 2.4% and 96.8% (gated on CD45+), respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Name
Catalog #
17956RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17956RF
Lot #
All
Language
French
Document Type
Product Name
Catalog #
17956
Lot #
All
Language
French
Document Type
Product Name
Catalog #
17956
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17956RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17956RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17956RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17956
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17956
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (9)

Brochure
Brochure
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll? or Lymphoprep?)
1:37
Video
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll? or Lymphoprep...
EasySep?のご説明 - 迅速かつ簡便な免疫磁気細胞分離テクノロジー
1:57
Video
EasySep?のご説明 - 迅速かつ簡便な免疫磁気細胞分離テクノロジー
How EasySep? Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep? Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Isolate Cells with a Simple Pour-Off: EasySep? Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep? Cell Separation Technology
How to Isolate Nucleated Cells from Whole Blood Using HetaSep? Erythrocyte Aggregation Agent
2:10
Video
How to Isolate Nucleated Cells from Whole Blood Using HetaSep? Erythrocyte Aggregation Agent

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