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EasySep? Human Central and Effector Memory CD8+ T Cell Isolation Kit

Immunomagnetic cell isolation kit using particle release technology

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EasySep? Human Central and Effector Memory CD8+ T Cell Isolation Kit

Immunomagnetic cell isolation kit using particle release technology

Catalog #
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Immunomagnetic cell isolation kit using particle release technology
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Product Advantages


  • Easy-to-use and column-free

  • Up to 93% purity

  • No-wash removal of EasySep? Releasable RapidSpheres?

What's Included

  • EasySep? Human Central and Effector Memory CD8+ T Cell Isolation Kit (Catalog #17869)
    • EasySep? Human Memory CD8+ T Cell Isolation Cocktail, 0.5 mL
    • EasySep? Dextran RapidSpheres?, 1 mL
    • EasySep? Human CD62L Positive Selection Cocktail, 1 mL
    • EasySep? Releasable RapidSpheres?, 1 mL
    • EasyeSep? Release Buffer, 1 mL
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. EasySep™ Magnet
    EasySep? Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep? Buffer

    Cell separation buffer

Overview

This kit isolates human CD62L+CD45RO+ and CD62L-CD45RO+ memory CD8+ T cells from fresh peripheral blood mononuclear cells (PBMCs) or washed leukapheresis samples. First, CD62L+ cells are isolated by column-free immunomagnetic positive selection using antibody complexes and EasySep? Releasable RapidSpheres?. Then, bound magnetic particles are removed from the EasySep?-isolated CD62L+ cells, and unwanted CD45RA+ and non-CD8+ T cells are targeted for depletion using antibody complexes and EasySep? Dextran RapidSpheres?. The final isolated fraction contains highly purified CD62L+CD45RO+ central memory CD8+ T cells that are immediately ready for downstream applications. Effector memory CD8+ T cells can be isolated from the CD62L- fraction.
Magnet Compatibility
? EasySep? Magnet (Catalog #18000)
? “The Big Easy” EasySep? Magnet (Catalog #18001)
? Easy 50 EasySep? Magnet (Catalog #18002)
? EasyEights? EasySep? Magnet (Catalog #18103)
Subtype
Cell Isolation Kits
Cell Type
T Cells, CD8+
Species
Human
Sample Source
Leukapheresis, PBMC
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology

Data Figures

Starting with fresh PBMCs, the central memory CD8+ T cell content (CD3+CD8+CD45RO+CD62L+) of the isolated fraction is typically 86.8 ± 8.4% (mean ± SD using the purple EasySep? magnet). The effector memory content (CD3+CD8+CD45RO+CD62L-) of the isolated fraction is typically 88.7 ± 6.5% (mean ± SD).
NOTE: RBCs were removed from the start sample by lysis prior to flow cytometry.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Name
Catalog #
17869
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17869
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17869
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17869
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17869
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17869
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17869
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (9)

Brochure
Brochure
Human Immune Cytokines
Wallchart
Human Immune Cytokines
How EasySep? Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep? Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll? or Lymphoprep?)
1:37
Video
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll? or Lymphoprep...
EasySep?のご説明 - 迅速かつ簡便な免疫磁気細胞分離テクノロジー
1:57
Video
EasySep?のご説明 - 迅速かつ簡便な免疫磁気細胞分離テクノロジー
Isolate Cells with a Simple Pour-Off: EasySep? Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep? Cell Separation Technology
Cancer Cells Impair T Cell Metabolism
41:01
Webinar
Cancer Cells Impair T Cell Metabolism
Scientific Poster

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Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT 海角破解版, REFER TO WWW.海角破解版.COM/COMPLIANCE.

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