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EasySep? Human Central and Effector Memory CD4+ T Cell Isolation Kit

Immunomagnetic cell isolation kit using particle release technology

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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

EasySep? Human Central and Effector Memory CD4+ T Cell Isolation Kit

Immunomagnetic cell isolation kit using particle release technology

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Immunomagnetic cell isolation kit using particle release technology
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Product Advantages


  • Easy-to-use and column-free

  • Up to 96% purity

  • No-wash removal of EasySep? Releasable RapidSpheres?

What's Included

  • EasySep? Human Central and Effector Memory CD4+ T Cell Isolation Kit (Catalog #17865)
    • EasySep? Human Memory CD4+ T Cell Isolation Cocktail, 0.5 mL
    • EasySep? Dextran RapidSpheres?, 1 mL
    • EasySep? Human CD62L Positive Selection Cocktail, 1 mL
    • EasySep Human CD25 Depletion Cocktail, 1 mL
    • EasySep? Releasable RapidSpheres?, 1 mL
    • EasyeSep? Release Buffer, 1 mL
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. EasySep™ Magnet
    EasySep? Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep? Buffer

    Cell separation buffer

Overview

This kit isolates human CD62L+CD45RO+ and CD62L-CD45RO+ memory CD4+ T cells from fresh peripheral blood mononuclear cells (PBMCs) or washed leukapheresis samples. First, CD62L+ cells are isolated by column-free immunomagnetic positive selection using antibody complexes and EasySep? Releasable RapidSpheres?. Then, bound magnetic particles are removed from the EasySep?-isolated CD62L+ cells, and unwanted CD45RA+ and non-CD4+ T cells are targeted for depletion using antibody complexes and EasySep? Dextran RapidSpheres?. The final isolated fraction contains highly purified CD62L+CD45RO+ central memory CD4+ T cells that are immediately ready for downstream applications. Effector memory CD4+ T cells can be isolated from the CD62L- fraction.
Magnet Compatibility
? EasySep? Magnet (Catalog #18000)
? “The Big Easy” EasySep? Magnet (Catalog #18001)
? Easy 50 EasySep? Magnet (Catalog #18002)
? EasyEights? EasySep? Magnet (Catalog #18103)
Subtype
Cell Isolation Kits
Cell Type
T Cells, CD4+
Species
Human
Sample Source
Leukapheresis, PBMC
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology

Data Figures

Starting with fresh PBMCs, the central memory CD4+ T cell content (CD3+CD4+CD45RO+CD62L+) of the isolated fraction is typically 92.3 ± 3.9% (mean ± SD using the purple EasySep? magnet). The effector memory content (CD3+CD4+CD45RO+CD62L-) of the isolated fraction is typically 92.4 ± 4.1% (mean ± SD).
NOTE: RBCs were removed from the start sample by lysis prior to flow cytometry.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Name
Catalog #
17865
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17865
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17865
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17865
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17865
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17865
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17865
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17865
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (10)

Brochure
Brochure
Frequencies and Percentages of Mouse Immune Cell Types
Wallchart
Frequencies and Percentages of Mouse Immune Cell Types
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Antigen Processing and Presentation
Wallchart
Antigen Processing and Presentation
How EasySep? Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep? Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll? or Lymphoprep?)
1:37
Video
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll? or Lymphoprep...
EasySep?のご説明 - 迅速かつ簡便な免疫磁気細胞分離テクノロジー
1:57
Video
EasySep?のご説明 - 迅速かつ簡便な免疫磁気細胞分離テクノロジー
Isolate Cells with a Simple Pour-Off: EasySep? Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep? Cell Separation Technology
Scientific Poster

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