海角破解版

  • Compare Products
Menu
Account
Make more informed purchasing decisions with our new product availability and delivery estimate feature, now available on all product pages, in your cart, and during checkout.

Easy 250 EasySep? Magnet

Magnet for large-scale immunomagnetic separation

Loading...
 
 

Easy 250 EasySep? Magnet

Magnet for large-scale immunomagnetic separation

Catalog #
(Select a product)
Magnet for large-scale immunomagnetic separation
Request Pricing

Product Advantages


  • EFFICIENT. Increase your cell isolation throughput by isolating cells from large-volume samples in a single separation.

  • FAST. Isolate immune cells from samples up to 225 mL in as little as 20 minutes.

  • HIGH PURITY. Achieve up to 99% cell purities of viable and functional cells.

  • FLEXIBLE. Obtain your specific cell type of interest with a wide range of compatible EasySep? Cell Isolation Kits.

Overview

Easily and efficiently scale up your manual cell isolations from large-volume samples, such as leukopaks and whole blood. Rather than splitting your cell suspension and performing multiple rounds of isolations, with the Easy 250 EasySep? Magnet, you can process up to 225 mL and 1.25 x 1010cells in a single isolation.

Use the Easy 250 EasySep? Magnet with a standard T-75 cm2 cell culture flask and EasySep? reagents to separate cells from large-volume samples without the use of columns. With its strong, high-gradient internal magnetic field, the magnet isolates cells labeled with magnetic EasySep? particles, allowing you to simply pipette out the unlabeled cell fraction and obtain cells that are immediately ready for downstream applications. Save time and obtain highly purified cells with EasySep? column-free technology.

Not sure which magnet to use? Visit our EasySep? Cell Separation Magnets page to compare the different options and select the appropriate magnet for your research.

Learn more about how immunomagnetic works.
Species
Human
Application
Cell Isolation
Brand
EasySep

Data Figures

Figure 1. Using the Easy 250 EasySep? Magnet Results in the Isolation of Highly Purified Cells of Interest

Human immune cells were isolated from processed leukopaks using the corresponding (A) EasySep? negative selection kits or (B) EasySep? positive selection kits (see Table 1). Cell purity was measured before isolation (Start) and after isolation with the Easy 250 EasySep? Magnet (Easy 250), EasySep? Magnet (Purple) or “The Big Easy” EasySep? Magnet (Silver). Purity was assessed by staining with cell surface markers for pan-T cells (CD3+), CD4+ T cells (CD3+CD4+), CD8+ T cells (CD3+CD8+), or monocytes (CD14+CD45+), and analyzed by flow cytometry. Data shown as mean ± SD; n = 4 - 12.

Figure 2. Easy 250 EasySep? Magnet Can Be Used to Isolate Mononuclear Cells from Whole Blood and Leukopak Samples of Up to 125 mL

Mononuclear cells (MNCs) were isolated using the EasySep? Direct Human PBMC Isolation Kit (Catalog #19654) from large whole blood samples (25 - 125 mL) or unprocessed leukopaks (25 - 125 mL) with the Easy 250 EasySep? Magnet (Easy 250) and from small samples (1 mL whole blood) with the EasySep? Magnet (Purple). (A) Representative flow cytometry plots show the MNC fraction (Glycophorin A- CD42b-). In the above example, the mononuclear cell content of the whole blood start sample (lysed by ammonium chloride) and non-lysed final isolated fraction is 28.9% and 97.1% (not gated on CD45), respectively. (B) Cell purity was measured before (Start) and after isolation (Easy 250, Purple) based on viable cells. Cells were counted and analyzed by flow cytometry. Data shown as mean ± SD; n = 7 - 9.

Typical Easy 250 EasySep? Magnet Cell Isolation Protocol (Negative Selection)

Figure 3. Typical Easy 250 EasySep? Magnet Cell Isolation Protocol (Negative Selection)

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Name
Catalog #
100-0821
Lot #
All
Language
English

Applications

Resources and Publications

Educational Materials (17)

Brochure
Brochure
Brochure
Brochure
How to Process a Leukopak for Downstream Cell Isolation
Protocol
How to Process a Leukopak for Downstream Cell Isolation
How EasySep? Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep? Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
A Novel Magnetic Platform for Large-Volume Cell Isolation from Whole Blood and Leukopaks
4:08
Video
A Novel Magnetic Platform for Large-Volume Cell Isolation from Whole Blood and Leukopaks
EasySep?のご説明 - 迅速かつ簡便な免疫磁気細胞分離テクノロジー
1:57
Video
EasySep?のご説明 - 迅速かつ簡便な免疫磁気細胞分離テクノロジー
How to Isolate Immune Cells from Large-Volume Samples Using the Easy 250 EasySep? Magnet
14:21
Video
How to Isolate Immune Cells from Large-Volume Samples Using the Easy 250 EasySep? Magnet
Getting Started with the Easy 250 EasySep? Magnet
2:54
Video
Getting Started with the Easy 250 EasySep? Magnet
Scale Up Your Cell Isolation
0:41
Video
Scale Up Your Cell Isolation
Key Regulatory Considerations for Moving Your Cell Therapy Research to the Clinic
28:34
Webinar
Key Regulatory Considerations for Moving Your Cell Therapy Research to the Clinic
Quick and Easy Isolation of T Cells and Other Immune Cells from Large-Volume Samples
37:09
Webinar
Quick and Easy Isolation of T Cells and Other Immune Cells from Large-Volume Samples
Next-Generation Cell Isolation Solutions for T Cell Therapy Research
27:28
Webinar
Next-Generation Cell Isolation Solutions for T Cell Therapy Research
How to Optimize Your T Cell Therapy Workflow—Without the Use of Serum or Feeder Cells
27:26
Webinar
How to Optimize Your T Cell Therapy Workflow—Without the Use of Serum or Feeder Cells
Scientific Poster
Human Immune Cell Isolation Course
On-Demand Training
Human Immune Cell Isolation Course

Related Products

Related Products
Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT 海角破解版, REFER TO WWW.海角破解版.COM/COMPLIANCE.

Copyright ? 2025 海角破解版. All rights reserved.