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The 30-F11 antibody reacts with an extracellular epitope found on all isoforms and both alloantigens (CD45.1 and CD45.2) of mouse CD45, a type I transmembrane glycoprotein expressed on the surface of most hematopoietic cells except mature erythrocytes, platelets and plasma cells; expression of CD45 is lost during differentiation of these cell types. CD45 is a member of the protein tyrosine phosphatase family and functions in a number of immunoregulatory processes, including cell activation, growth, differentiation and oncogenic transformation. The large cytoplasmic portion of CD45 contains two tyrosine phosphatase domains, one which is enzymatically active, that are involved in modulating the function of intracellular substrates such as the Src kinases Lck and Fyn. Several isoforms of CD45 have been identified, the expression of which differs according to cell type and functional status. Alternative splicing of three exons (4, 5, 6) encoding the extracellular RA, RB and RC polypeptide sequences gives rise to up to 8 isoforms with molecular masses in the range of 180 - 240 kDa.
Subtype
Primary Antibodies
Target Antigen
CD45
Alternative Names
LCA, Leukocyte common antigen, Ly-5, Protein tyrosine phosphatase receptor type C, PTPRC, T200
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45 Antibody, Clone 30-F11, followed by a mouse anti-rat IgG2b antibody, FITC (filled histogram), or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530 (Catalog #60077), followed by a mouse anti-rat IgG2b antibody, FITC (solid line histogram).
Figure 2. Data for Alexa Fluor® 488-Conjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45 Antibody, Clone 30-F11, Alexa Fluor® 488 (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, Alexa Fluor® 488 (Catalog #60077AD) (solid line histogram).
Figure 3. Data for APC-Conjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45 Antibody, Clone 30-F11, APC (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, APC (Catalog #60077AZ) (solid line histogram).
Figure 4. Data for Biotin-Conjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45 Antibody, Clone 30-F11, Biotin, followed by streptavidin (SAV) APC (filled histogram), or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, Biotin (Catalog #60077BT), followed by SAV APC (solid line histogram).
Figure 5. Data for FITC-Conjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45 Antibody, Clone 30-F11, FITC (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, FITC (Catalog #60077FI) (solid line histogram).
Figure 6. Data for PE-Conjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45 Antibody, Clone 30-F11, PE (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, PE (Catalog #60077PE) (solid line histogram).
Figure 7. Data for PerCP-Cy55-Conjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45 Antibody, Clone 30-F11, PerCP-Cy5.5 (filled histogram) or a rat IgG2b, kappa PerCP-Cy5.5 isotype control antibody (solid line histogram).
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Rat monoclonal IgG2b, kappa isotype control antibody
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Anti-Mouse CD45 Antibody, Clone 30-F11
Legal Statement:
Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT º£½ÇÆƽâ°æ, REFER TO WWW.º£½ÇÆƽâ°æ.COM/COMPLIANCE.
