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Anti-Mouse CD4 Antibody, Clone RM4-4

Rat monoclonal IgG2b antibody against mouse CD4

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Anti-Mouse CD4 Antibody, Clone RM4-4

Rat monoclonal IgG2b antibody against mouse CD4

Catalog #
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Rat monoclonal IgG2b antibody against mouse CD4
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Overview

The RM4-4 antibody reacts with mouse CD4, an ~55 kDa single-chain type 1 transmembrane glycoprotein and member of the immunoglobin (Ig) superfamily; CD4 contains four extracellular Ig-like domains. CD4 is expressed at relatively high levels by most thymocytes and a subpopulation of T cells (T-helper/inducer cells), and at lower levels on dendritic cells. In the mouse, CD4 is not expressed by monocytes/macrophages. CD4 binds to a non-polymorphic region of MHC II and acts as a co-receptor to the T cell receptor (TCR) in MHC II-restricted antigen recognition by enhancing the avidity of the association between the TCR and MHC II-antigen complex. CD4 also functions to amplify signals from the TCR to the cytoplasm through the interaction of its intracellular domain with cytoplasmic tyrosine kinases such as Lck. Binding of the RM4-4 antibody does not block binding of antibody clones RM4-5 or GK1.5.
Subtype
Primary Antibodies
Target Antigen
CD4
Alternative Names
L3T4, T4
Reactive Species
Mouse
Conjugation
APC, Biotin, FITC, PE, Unconjugated
Host Species
Rat
Cell Type
T Cells, T Cells, CD4+, T Cells, Regulatory
Species
Mouse
Application
Flow Cytometry, Functional Assay
Area of Interest
Immunology
Clone
RM4-4
Gene ID
12504
Isotype
IgG2b, kappa

Data Figures

Data for Unconjugated

Figure 1. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-4, followed by a mouse anti-rat IgG2b antibody, FITC (filled histogram), or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530 (Catalog #60077), followed by a mouse anti-rat IgG2b antibody, FITC (solid line histogram).

Data for APC-Conjugated

Figure 2. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-4, APC (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, APC (Catalog #60077AZ) (solid line histogram).

Data for Biotin-Conjugated

Figure 3. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-4, Biotin, followed by streptavidin (SAV) APC (filled histogram), or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, Biotin (Catalog #60077BT), followed by SAV APC (solid line histogram).

Data for FITC-Conjugated

Figure 4. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-4, FITC (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, FITC (Catalog #60077FI) (solid line histogram).

Data for PE-Conjugated

Figure 5. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-4, PE (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, PE (Catalog #60077PE) (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Name
Catalog #
60029BT, 60029BT.1
Lot #
All
Language
English
Document Type
Product Name
Catalog #
60029BT, 60029BT.1
Lot #
All
Language
English
Document Type
Product Name
Catalog #
60029FI, 60029FI.1
Lot #
All
Language
English
Document Type
Product Name
Catalog #
60029PE.1, 60029PE
Lot #
All
Language
English
Document Type
Product Name
Catalog #
60029AZ, 60029AZ.1
Lot #
All
Language
English
Document Type
Product Name
Catalog #
60029BT, 60029BT.1
Lot #
All
Language
English
Document Type
Product Name
Catalog #
60029FI, 60029FI.1
Lot #
All
Language
English
Document Type
Product Name
Catalog #
60029PE.1, 60029PE
Lot #
All
Language
English
Document Type
Product Name
Catalog #
60029AZ, 60029AZ.1
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (4)

Brochure
Brochure
Brochure
Human Immune Cytokines
Wallchart
Human Immune Cytokines

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Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT º£½ÇÆƽâ°æ, REFER TO WWW.º£½ÇÆƽâ°æ.COM/COMPLIANCE.

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