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The 3G8 antibody reacts with CD16 (Fc纬RIII or low affinity IgG receptor III), a type 1 transmembrane glycoprotein belonging to the Ig superfamily. CD16 exists in two distinct isoforms: CD16a, a 50 - 65 kDa form expressed on NK cells, activated monocytes, macrophages, and placental trophoblasts, and CD16b, an ~48 kDa glycosylphosphatidylinositol (GPI)-anchored form expressed on neutrophils, basophils and eosinophils and found as at least two polymorphic variants, termed NA1 and NA2. CD16 binds weakly to the Fc region of monomeric, aggregated or complexed IgG, particularly the IgG1 and IgG3 isotypes. Binding of IgG to either CD16 isoform induces signaling pathways that modulate several types of responses, including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, cytokine release and proliferation. CD16/IgG interactions can result in non-specific labeling in antibody-based detection and cell separation experiments and the 3G8 antibody may be employed as a blocking antibody to reduce non-specific binding.
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD16 Antibody, Clone 3G8, followed by a rat anti-mouse IgG1 antibody, PE (filled histogram), or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21 (Catalog #60070), followed by a rat anti-mouse IgG1 antibody, PE (solid line histogram).
Figure 2. Data for Alexa Fluor® 488-Conjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD16 Antibody, Clone 3G8, Alexa Fluor庐 488 (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Alexa Fluor庐 488 (Catalog #60070AD) (solid line histogram).
Figure 3. Data for APC-Conjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD16 Antibody, Clone 3G8, APC (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, APC (Catalog #60070AZ) (solid line histogram).
Figure 4. Data for Biotin-Conjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD16 Antibody, Clone 3G8, Biotin, followed by streptavidin (SAV) APC (filled histogram), or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Biotin (Catalog #60070BT), followed by SAV APC (solid line histogram).
Figure 5. Data for FITC-Conjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD16 Antibody, Clone 3G8, FITC (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, FITC (Catalog #60070FI) (solid line histogram).
Figure 6. Data for PE-Conjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD16 Antibody, Clone 3G8, PE (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PE (Catalog #60070PE) (solid line histogram).
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Mouse monoclonal IgG1, kappa isotype control antibody
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Anti-Human CD16 Antibody, Clone 3G8
Legal Statement:
Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor庐 are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT 海角破解版, REFER TO WWW.海角破解版.COM/COMPLIANCE.
