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EasySep™ Rat B Cell Isolation Kit

Immunomagnetic negative selection kit

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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

EasySep™ Rat B Cell Isolation Kit

Immunomagnetic negative selection kit

Catalog #
(Select a product)
Immunomagnetic negative selection kit
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Product Advantages


  • Fast, easy-to-use and column-free

  • Up to 95% purity

  • Isolated cells are untouched

What's Included

EasySep™ Rat B Cell Isolation Kit (Catalog #19644)
    • EasySep™ Rat B Cell Isolation Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50102, 1 mL
RoboSep™ Rat B Cell Isolation Kit (Catalog #19644RF)
    • EasySep™ Rat B Cell Isolation Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50102, 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

Overview

The EasySep™ Rat B Cell Isolation Kit is designed to isolate B cells from single-cell suspensions of splenocytes or lymph nodes or from whole blood by negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• ”The BigEasy” EasySep™ Magnet (Catalog #18001)
• Easy50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
B Cells
Species
Rat
Sample Source
Other, Spleen, Whole Blood
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology

Data Figures

19644 FACS

Starting with a single-cell suspension of rat splenocytes, the B cell content (CD45RA+CD3-) of the isolated fraction is typically 94.1 ± 3.8% (mean ± SD using the purple EasySep™ Magnet). In the example above, the purities of the start and final isolated fractions are 44.5% and 95.6%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Name
Catalog #
19644
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19644RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19644
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19644
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19644RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19644RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
19644RF
Lot #
All
Language
English

Resources and Publications

Educational Materials (8)

Brochure
Brochure
Brochure
Frequencies of Immune Cells in Rat Tissue
Wallchart
Frequencies of Immune Cells in Rat Tissue
Human Immune Cytokines
Wallchart
Human Immune Cytokines
How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology
1:23
Video
How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
1:41
Video
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument

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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
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