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EasySep? HLA Chimerism Whole Blood CD19 Positive Selection Kit

Immunomagnetic positive selection of human CD19+ cells from whole blood

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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

EasySep? HLA Chimerism Whole Blood CD19 Positive Selection Kit

Immunomagnetic positive selection of human CD19+ cells from whole blood

Catalog #
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Immunomagnetic positive selection of human CD19+ cells from whole blood
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Product Advantages


  • Fast and easy-to-use

  • Up to 99% purity

  • No columns required

  • Compatible across EasySep? "The Big Easy", EasyEights?, and RoboSep?-S platforms

What's Included

  • EasySep? HLA Chimerism Whole Blood CD19 Positive Selection Kit (Catalog #17874)
    • EasySep? HLA Chimerism Whole Blood CD19 Positive Selection Cocktail, 3 x 1 mL
    • EasySep? Dextran RapidSpheres? 50101, 3 x 1 mL
    • EasySep? RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
  • RoboSep? HLA Chimerism Whole Blood CD19 Positive Selection Kit (Catalog #17874RF)
    • EasySep? HLA Chimerism Whole Blood CD19 Positive Selection Cocktail, 3 x 1 mL
    • EasySep? Dextran RapidSpheres? 50101, 3 x 1 mL
    • EasySep? RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
    • RoboSep? Buffer (Catalog #20104) x 2
    • RoboSep? Filter Tips (Catalog #20125) x 2
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. The Big Easy EasySep™ Magnet
    "The Big Easy" EasySep? Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep? Buffer

    Cell separation buffer

Overview

Isolate highly purified human CD19+ cells from whole blood or buffy coat and leukoreduction system chamber (LRSC; also known as an LRS cone) samples by immunomagnetic positive selection, with the EasySep? HLA Chimerism Whole Blood CD19 Positive Selection Kit. Widely used in published research for more than 20 years, EasySep? combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep? positive selection procedure, desired cells are labeled with antibody complexes recognizing CD19 and magnetic particles. The cocktail in this kit also contains an antibody to human Fc receptor to prevent non-specific binding. Labeled cells are separated using an EasySep? magnet and by simply pouring or pipetting off the unwanted cells. The cells of interest remain in the tube. Following magnetic cell isolation, the desired CD19+ cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction. The CD19 antigen is present on mature B cells and B cell progenitors but is lost upon maturation to plasma cells. It is also expressed on follicular dendritic cells.

This product replaces the EasySep? Human Whole Blood CD19 Positive Selection Kit (Catalog #18084) for even faster cell isolations.

Learn more about how immunomagnetic EasySep? technology works or how to fully automate immunomagnetic cell isolation with RoboSep? to save time and increase laboratory throughput. Explore additional products optimized for your workflow, including those for cell characterization, cryopreservation, and more.

Magnet Compatibility
? “The Big Easy” EasySep? Magnet (Catalog #18001)
? EasyEights? EasySep? Magnet (Catalog #18103)
? RoboSep?-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
B Cells
Species
Human
Sample Source
Buffy Coat, Whole Blood
Selection Method
Positive
Brand
EasySep
Area of Interest
HLA, Immunology

Data Figures

Typical EasySep? HLA Chimerism Whole Blood CD19 Positive Selection Profile

Figure 1. Typical EasySep? HLA Chimerism Whole Blood CD19 Positive Selection Profile

Starting with human whole blood, the CD19+ cell content of the isolated fraction typically ranges from 94.3 - 99.6% (as assessed by staining the start and isolated fractions with anti-CD19 or anti-CD20 antibodies, respectively). In the above example, the purities of the start and the final isolated fractions are 5.0% and 98.6%, respectively (gated on CD45).
NOTE: Red blood cells were removed from the start sample by lysis prior to flow cytometry.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Name
Catalog #
17874RF
Lot #
1000129406 or higher
Language
English
Document Type
Product Name
Catalog #
17874RF
Lot #
1000129406 or higher
Language
English
Document Type
Product Name
Catalog #
17874
Lot #
1000129406 or higher
Language
English
Document Type
Product Name
Catalog #
17874
Lot #
1000129406 or higher
Language
English
Document Type
Product Name
Catalog #
17874RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17874RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17874RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17874RF
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17874
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17874
Lot #
All
Language
English
Document Type
Product Name
Catalog #
17874
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (16)

Brochure
Brochure
Brochure
Fully Automated, High-Purity Cell Isolation for Chimerism Labs
Technical Bulletin
Fully Automated, High-Purity Cell Isolation for Chimerism Labs
Purity Assessment by Flow Cytometry for Lineage-Specific Chimerism Analysis
Technical Bulletin
Purity Assessment by Flow Cytometry for Lineage-Specific Chimerism Analysis
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
EasySep?のご説明 - 迅速かつ簡便な免疫磁気細胞分離テクノロジー
1:57
Video
EasySep?のご説明 - 迅速かつ簡便な免疫磁気細胞分離テクノロジー
How to Recover Cells from a Leukocyte Reduction System (LRS) Cone
4:55
Video
How to Recover Cells from a Leukocyte Reduction System (LRS) Cone
Automate Cell Isolation with the RoboSep?-S Cell Separation Instrument
1:41
Video
Automate Cell Isolation with the RoboSep?-S Cell Separation Instrument
How to Prepare a Buffy Coat from Whole Blood
3:46
Video
How to Prepare a Buffy Coat from Whole Blood
How EasySep? Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep? Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
The Nuts and Bolts of Lineage Chimerism Testing
33:41
Webinar
The Nuts and Bolts of Lineage Chimerism Testing
Increasing the Sensitivity of Cytogenetic Analysis of Hematologic Malignancies Through Cell Enrichment
59:59
Webinar
Increasing the Sensitivity of Cytogenetic Analysis of Hematologic Malignancies Through Cell Enrichme...
Fully Automated Cell Separation for Chimerism Analysis with RoboSep?
56:02
Webinar
Fully Automated Cell Separation for Chimerism Analysis with RoboSep?
Scientific Poster

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