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Anti-Mouse F4/80 Antibody, Clone BM8

Rat monoclonal IgG2a antibody against human, mouse F4/80

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Anti-Mouse F4/80 Antibody, Clone BM8

Rat monoclonal IgG2a antibody against human, mouse F4/80

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Rat monoclonal IgG2a antibody against human, mouse F4/80
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Overview

The BM8 antibody reacts with the F4/80 antigen (also termed Ly-71 in mouse and EMR-1 in human), an ~160 kDa transmembrane glycoprotein belonging to the EGF-TM7 family of G-protein-coupled receptors. F4/80 is considered a marker of choice for the identification of mature tissue macrophages, being broadly but variably expressed by this cell type in the liver (Kupffer cells), skin (Langerhans cells), bone marrow stroma, pancreas, thymus, spleen (red pulp), lung, and other tissues. It is also expressed by circulating monocytes, eosinophils and a subset of dendritic cells. F4/80 expression levels increase following activation of macrophages. F4/80 is reportedly the only macrophage marker suitable for distinguishing destructive from non-destructive inflammatory processes in the pancreas. The protein is thought to play a role in the generation of CD8+ regulatory T cells involved in immune tolerance.

This antibody clone has been verified for purity assessments of cells isolated with EasySep鈩 kits, including EasySep鈩 Mouse Monocyte Enrichment Kit (Catalog #19761) and EasySep鈩 Mouse CD11b Positive Selection Kit (Catalog #18770).
Subtype
Primary Antibodies
Target Antigen
F4/80
Alternative Names
EMR1, Ly71
Reactive Species
Human, Mouse
Conjugation
Alexa Fluor 488, APC, Biotin, FITC, PE, PerCP-Cyanine5.5, Unconjugated
Host Species
Rat
Cell Type
Monocytes, Other
Species
Human, Mouse
Application
Cell Isolation, ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Area of Interest
Immunology
Clone
BM8
Gene ID
13733
Isotype
IgG2a, kappa

Data Figures

Data for Alexa Fluor® 488-Conjugated

Figure 1. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse F4/80 Antibody, Clone BM8, Alexa Fluor® 488 and anti-mouse CD45 APC.
(B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells processed with the EasySep™ Mouse CD11b Positive Selection Kit and labeled with Anti-Mouse F4/80 Antibody, Clone BM8, Alexa Fluor® 488.
(C) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with a rat IgG2a, kappa Alexa Fluor® 488 isotype control antibody and antimouse CD45 APC.

Data for PE-Conjugated

Figure 2. Data for PE-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse F4/80 Antibody, Clone BM8, PE and anti-mouse CD45 APC.
(B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with a rat IgG2a, kappa PE isotype control antibody and anti-mouse CD45 APC.

Data for Unconjugated

Figure 3. Data for Unconjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse F4/80 Antibody, Clone BM8, followed by a mouse anti-rat IgG2a antibody, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758 (Catalog #60076), followed by a mouse anti-rat IgG2a antibody, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.

Data for APC-Conjugated

Figure 4. Data for APC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse F4/80 Antibody, Clone BM8, APC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE (Catalog #60019PE). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, APC (Catalog #60076AZ) and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE.

Data for Biotin-Conjugated

Figure 5. Data for Biotin-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse F4/80 Antibody, Clone BM8, Biotin, followed by streptavidin (SAV) APC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE (Catalog #60019PE). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Biotin (Catalog #60076BT), followed by SAV APC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE.

Data for FITC-Conjugated

Figure 6. Data for FITC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse F4/80 Antibody, Clone BM8, FITC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE (Catalog #60019PE). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, FITC (Catalog #60076FI) and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE.

Data for PerCP-Cy55-Conjugated

Figure 7. Data for PerCP-Cy55-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse F4/80 Antibody, Clone BM8, PerCP-Cy5.5 and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE (Catalog #60019PE). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with a rat IgG2a, kappa isotype control antibody, PerCP-Cy5.5 and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Name
Catalog #
60027PE, 60027PE.1
Lot #
All
Language
English
Document Type
Product Name
Catalog #
60027
Lot #
All
Language
English
Document Type
Product Name
Catalog #
60027BT, 60027BT.1
Lot #
All
Language
English
Document Type
Product Name
Catalog #
60027FI.1, 60027FI
Lot #
All
Language
English
Document Type
Product Name
Catalog #
60027AD.1, 60027AD
Lot #
All
Language
English
Document Type
Product Name
Catalog #
60027PS.1, 60027PS
Lot #
All
Language
English
Document Type
Product Name
Catalog #
60027AZ.1, 60027AZ
Lot #
All
Language
English
Document Type
Product Name
Catalog #
60027PE, 60027PE.1
Lot #
All
Language
English
Document Type
Product Name
Catalog #
60027
Lot #
All
Language
English
Document Type
Product Name
Catalog #
60027BT, 60027BT.1
Lot #
All
Language
English
Document Type
Product Name
Catalog #
60027FI.1, 60027FI
Lot #
All
Language
English
Document Type
Product Name
Catalog #
60027AD.1, 60027AD
Lot #
All
Language
English
Document Type
Product Name
Catalog #
60027PS.1, 60027PS
Lot #
All
Language
English
Document Type
Product Name
Catalog #
60027AZ.1, 60027AZ
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (3)

Brochure
Brochure
Human Immune Cytokines
Wallchart
Human Immune Cytokines

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Legal Statement:

Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor庐 are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT 海角破解版, REFER TO WWW.海角破解版.COM/COMPLIANCE.

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